TNF-α和TGF-β1在胚胎停育患者蜕膜组织中的表达  

Expression of TNF-αand TGF-β1 in decidua tissue of patients with embryo damage

作　　者：崔莉 刘杰 邹歌 王怡佳 陈燕 董建新 CUI Li;LIU Jie;ZOU Ge;WANG Yijia;CHEN Yan;DONG Jianxin(Department of Obstetrics and Gynecology,North China University of Science and Technology Affiliated Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]华北理工大学附属医院妇产科,河北唐山063000

出　　处：《重庆医学》2024年第6期861-865,871,共6页Chongqing medicine

基　　金：2022年度河北省医学科学研究课题计划项目(20221548)。

摘　　要：目的 探讨蜕膜组织肿瘤坏死因子-α(TNF-α)和转化生长因子-β1(TGF-β1)表达与胚胎停育的关系。方法 选取2020年11月至2021年10月就诊于该院妇产科门诊经B超确诊为胚胎停育的患者40例为观察组,另选取同期正常妊娠并要求人工流产的孕妇40例为对照组。采用免疫组织化学法与实时荧光定量逆转录PCR(RT-qPCR)检测两组蜕膜组织TNF-α和TGF-β1及其mRNA表达水平并比较。以两组蜕膜组织中TNF-α和TGF-β1 mRNA相对表达水平的均值为临界值将患者分为不同亚组,进一步分析TNF-α和TGF-β1 mRNA表达水平与胚胎停育的关系。结果 免疫组织化学结果显示,观察组蜕膜组织中TNF-α、TGF-β1阳性表达率分别为80.0%、 52.5%,对照组分别为47.5%、75.0%;观察组蜕膜组织中TNF-α表达程度高于对照组,TGF-β1表达程度低于对照组,差异均有统计学意义(Z=-3.622、3.898,P<0.05)。RT-qPCR结果显示,观察组蜕膜组织中TNF-α mRNA相对表达水平高于对照组(6.16±1.25 vs. 1.02±0.02),TGF-β1 mRNA相对表达水平低于对照组(0.59±0.19 vs. 1.03±0.03),差异均有统计学意义(t=26.144、-14.199,P<0.05);分别以蜕膜组织TNF-α和TGF-β1 mRNA相对表达水平3.59、0.81为临界值进行分析,TNF-α和TGF-β1 mRNA相对表达水平与胚胎停育的发生有关(OR=0.050,95%CI:0.013~0.193,P<0.001;OR=0.125,95%CI:0.055~0.284,P<0.001)。结论 蜕膜组织TNF-α表达升高,TGF-β1表达降低可能与胚胎停育有关。Objective To investigate the relationship between the expression of tumor necrosis factor-α(TNF-α)and transforming growth factor-β1(TGF-β1)in decidual tissue and embryo damage.Methods From November 2020 to October 2021,40 patients attending out-patient clinic of obstetrics and gynecology of the hospital diagnosed with embryo damage by B-ultrasound were selected as the observation group,and 40 normal pregnant women who required artificial abortion during the same period were selected as the control group.Immunohistochemistry and reverse transcription real-time quantitative PCR(RT-qPCR)were used to detect the expression levels of TNF-αand TGF-β1 and their mRNA in decidua tissues,and the expression levels were compared between the two groups.The patients were divided into different subgroups with the mean values of TNF-αand TGF-β1 mRNA expression in decidua tissues as critical values,and the relationship between TNF-αand TGF-β1 mRNA expression levels and embryo damage was further analyzed.Results Immunohistochemistry results showed that the positive expression rates of TNF-αand TGF-β1 in decidua tissues were 80.0%and 52.5%in the observation group,and 47.5%and 75.0%in the control group,respectively.The expression of TNF-αin decidua tissues of the observation group was stronger than that of the control group,the expression of TGF-β1 in decidua tissues of the observation group was weaker than that of the control group,and the differences were statistically significant(Z=-3.622,3.898,P<0.05).RT-qPCR results showed that the relative expression level of TNF-αmRNA in decidua tissues of the observation group was higher than that of the control group(6.16±1.25 vs.1.02±0.02),while the relative expression level of TGF-β1 mRNA of the observation group was lower than that of the control group(0.59±0.19 vs.1.03±0.03),and the differences were statistically significant(t=26.144,-14.199,P<0.05).The relative expression levels of TNF-αand TGF-β1 mRNA in decidua tissues of the two groups were analyzed with the cri

关 键 词：胚胎停育 蜕膜组织 肿瘤坏死因子-Α 转化生长因子-Β1 免疫组织化学法 实时荧光定量逆转录PCR 

分 类 号：R714.21[医药卫生—妇产科学]