Ang-1和MCP-1在小儿支气管哮喘外周血中的表达及其与肺功能的关系  

Expression of Ang-1 and MCP-1 in Peripheral Blood of Children with Bronchial Asthma and Their Correlation with Lung Function

作　　者：孙灿光 张弦 颜晓磊 岳文静 SUN Canguang;ZHANG Xian;YAN Xiaolei;YUE Wenjing(Department of Respiratory Medicine,Kaifeng Children’s Hospital,Kaifeng 475000,China;Department of Health Care,Kaifeng Children’s Hospital,Kaifeng 475000,China;Department of Gastroenterology,Kaifeng Children’s Hospital,Kaifeng 475000,China;Department of Hematology,Kaifeng Children’s Hospital,Kaifeng 475000,China)

机构地区：[1]开封市儿童医院呼吸内科,河南开封475000 [2]开封市儿童医院保健科,河南开封475000 [3]开封市儿童医院消化内科,河南开封475000 [4]开封市儿童医院血液内科,河南开封475000

出　　处：《河南医学研究》2024年第5期885-888,共4页Henan Medical Research

摘　　要：目的探讨血管生成素-1(Ang-1)和趋化蛋白-1(MCP-1)在小儿支气管哮喘外周血中的表达及其与肺功能的关系。方法选取开封市儿童医院在2019年5月至2022年5月诊治的162例支气管哮喘患儿作为研究对象,设为研究组,依据全球哮喘防治倡议分为轻度持续组(92例)、中度持续组(37例)、重度持续组(33例),同时纳入80例健康体检者作为对照组。采用酶联免疫吸附试验测定MCP-1和Ang-1水平,采用肺功能仪检测各组儿童肺功能[第1秒用力呼气容积与用力肺活量比值(FEV 1/FVC)、第1秒用力呼气容积(FEV 1)、最大呼气流速(PEF)]。应用Pearson相关分析探讨Ang-1和MCP-1外周血水平与肺功能的相关性。结果哮喘患儿外周血Ang-1表达水平低于对照组,MCP-1水平高于对照组(P<0.05)。中、重度患儿外周血Ang-1水平低于轻度患儿(重度<中度<轻度),MCP-1水平均高于轻度患儿(重度>中度>轻度)(P<0.05)。与对照组相比,哮喘患儿FEV 1/FVC、FEV 1、PEF等检测指标均降低(P<0.05);中、重度哮喘患儿FEV 1/FVC、FEV 1、PEF等指标均低于轻度患者(重度<中度<轻度)(P<0.05)。哮喘患儿外周血中Ang-1与FEV 1/FVC、FEV 1、PEF等肺功能指标呈正相关(r=0.753、0.784、0.605,P<0.05),MCP-1与FEV 1/FVC、FEV 1、PEF等肺功能指标呈负相关(r=-0.724、-0.815、-0.618,P<0.05);Ang-1、MCP-1诊断儿童支气管哮喘的曲线下面积及95%CI分别是0.801(0.746~0.856)、0.803(0.778~0.828),诊断的cut-off值分别是17.88μg·L-1、58.47 ng·L-1,Ang-1诊断小儿支气管哮喘的灵敏度和特异度为85.54%和76.25%,MCP-1诊断小儿支气管哮喘的灵敏度和特异度为83.84%和77.50%,Ang-1和MCP-1联合检测可将灵敏度提高至89.50%。结论小儿支气管哮喘外周血Ang-1呈低表达,MCP-1呈高表达,前者与肺功能呈正相关,后者呈负相关,Ang-1和MCP-1对小儿支气管哮喘具有一定的诊断价值。Objective To investigate the expression of angiopoietin-1(Ang-1)and chemoattractant protein-1(MCP-1)in the peripheral blood of bronchial asthma children and their correlation with lung function.Methods A total of 162 children with bronchial asthma diagnosed and treated in Kaifeng Children’s Hospital from May 2019 to May 2022 were selected as the research object and set as the research group.According to the Global Initiative for Asthma,they were divided into mild persistent group(92 cases),moderate persistence group(37 cases)and severe persistence group(33 cases).At the same time,80 healthy subjects were included as the control group.The enzyme-linked immunosorbent was used to measure MCP-1 and Ang-1 levels,and pulmonary function meters were used to measure the lung function[forced expiratory volume in one second/forced vital capacity(FEV 1/FVC),forced expiratory volume in one second(FEV 1),peak expiratory flow rate(PEF)]of children in each group.The correlation between Ang-1 and MCP-1 peripheral blood levels and lung function were investigated by Pearson analysis.Results Compared with the control group,the expression level of Ang-1 in the peripheral blood of children with asthma was lower,and the level of MCP-1 was higher(P<0.05).Peripheral blood Ang-1 levels of the moderate and severe children were lower than those of the mild children(severe<moderate<mild),and MCP-1 levels were higher than those of the mild children(severe>moderate>mild)(P<0.05).Compared with the control group,the detection indicators of FEV 1/FVC,FEV 1,PEF and other indicators in children with asthma were lower(P<0.05).The indicators of FEV 1/FVC,FEV 1 and PEF in children with moderate and severe asthma were lower than those in mild patients(severe<moderate<mild)(P<0.05).Peripheral blood Ang-1 in children with asthma was positively correlated with lung function indexes such as FEV 1/FVC,FEV 1,PEF(r=0.753,0.784,0.605,P<0.05).MCP-1 was negatively correlated with FEV 1/FVC,FEV 1,PEF and other lung function indexes(r=-0.724,-0.815,-0.618,P<0.05

关 键 词：血管生成素-1 趋化蛋白-1 儿童 支气管哮喘 肺功能 

分 类 号：R725.6[医药卫生—儿科]