Recovery of cyanophycin granule polypeptide from activated sludge: carbon source dependence and aggregation-induced luminescence characteristics  

作　　者：Kui Zou Hongyuan Liu Bo Feng Taiping Qing Peng Zhang 

机构地区：[1]College of Environment and Resources,Xiangtan University,Xiangtan 411105,China

出　　处：《Frontiers of Environmental Science & Engineering》2024年第2期25-34,共10页环境科学与工程前沿（英文）

基　　金：supported by the National Natural Science Foundation of China(No.U22A20617);the Natural Science Foundation of Hunan Province,China(Nos.2021JJ30664,2022JJ30559,and 2022JJ50131);Environmental Protection Research Project of Hunan Province,China(No.HBKT-2022036).

摘　　要：In the sewage treatment process,facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic circulation.Cyanophycin granule polypeptide(CGP),a recently discovered high value-added biopolymer present in activated sludge,has provided new avenues for the recovery of resources.However,the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus far.This study investigated the synthesis of CGP,polyhydroxyalkanoates(PHA),and alginate-like exopolysaccharides(ALE)in various microbial aggregates under different carbon sources feeding conditions.Our results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding conditions.Furthermore,biofilm was more conducive to CGP accumulation than floc sludge.Compared with glucose and methanol,sodium acetate significantly enriched the CGP synthetase-encoding gene(cphAabundance=~17419),resulting in the highest CGP yield(average 107.1 mg/g MLSS)in both biofilm and floc sludge.This study is the first to reported the characteristic fluorescence of CGP(Ex/Em=~360/450 nm)caused by the aggregated luminescence of arginine on the side chains.Overall,this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.

关 键 词：Cyanophycin POLYHYDROXYALKANOATE Alginate-like exopolysaccharides Bioresource recovery Aggregation-induced luminescence 

分 类 号：X703[环境科学与工程—环境工程]