Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders  

作　　者：Luning Lian Yi Xing Dayi Zhang Longfei Jiang Mengke Song Bo Jiang 

机构地区：[1]School of Energy and Environmental Engineering,University of Science&Technology Beijing,Beijing 100083,China [2]Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants,University of Science&Technology Beijing,Beijing 100083,China [3]National Environmental and Energy Science and Technology International Cooperation Base,University of Science&Technology Beijing,Beijing 100083,China [4]College of New Energy and Environment,Jilin University,Changchun 130021,China [5]Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou 510640,China [6]College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China [7]National Engineering Laboratory for Site Remediation Technologies,Beijing 100015,China

出　　处：《Frontiers of Environmental Science & Engineering》2024年第1期59-69,共11页环境科学与工程前沿（英文）

基　　金：The authors would like to thank the National Natural Science Foundation of China(Nos.42177359 and 41807119);the Natural Science Foundation of Beijing(No.8212030);the Fundamental Research Funds for the Central Universities(Nos.FRF-TP-20-010A3 and FRF-IDRY-22-001);the Open Fund of National Engineering Laboratory for Site Remediation Technologies(No.NEL-SRT201907).

摘　　要：Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify the functional microorganisms in degrading dimethoate in agricultural soils. MMI identified five dimethoate degraders: Pseudomonas, Bacillus, Ramlibacter, Arthrobacter, and Rhodococcus, whereas DNA-SIP identified three dimethoate degraders: Ramlibacter, Arthrobacter, and Rhodococcus. Also, MMI showed higher resolution than DNA-SIP in identifying functional microorganisms. Two organic phosphohydrolase (OPH) genes: ophC2 and ophB, were involved in dimethoate metabolism, as revealed by DNA-SIP and MMI. The degradation products of dimethoate include omethoate, O,O,S-trimethyl thiophosphorothioate, N-methyl-2-sulfanylacetamide, O,O-diethyl S-hydrogen phosphorodithioate, O,O,O-trimethyl thiophosphate, O,O,S-trimethyl thiophosphorodithioate, and O,O,O-trimethyl phosphoric. This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders, expanding our knowledge of microbial resources with cultivation-independent approaches.

关 键 词：Stable isotope probing(SIP) Magnetic-nanoparticle mediated isolation(MMI) DIMETHOATE Biodegradation Cultivation-independent approach 

分 类 号：Q50[生物学—生物化学]