丹皮酚对大鼠卵母细胞体外成熟体系氧化应激的调控  

The regulatory effect of paeonol on oxidative stress in the rat oocytes in vitro maturation system

作　　者：张春强 田荆华[1] 王守福 赵辉[1] 李岩 郭新庆[1] 韦岩[1] 王海蓉[1] 刘胜[1] 薛兰 ZHANG Chunqiang;TIAN Jinghua;WANG Shoufu;ZHAO Hui;LI Yan;GUO Xinqing;WEI Yan;WANG Hairong;LIU Sheng;XUE Lan(Heze Medical College,Heze 274000,Shandong)

机构地区：[1]菏泽医学专科学校,山东菏泽274000

出　　处：《菏泽医学专科学校学报》2024年第1期11-16,共6页Journal of Heze Medical College

基　　金：山东省中医药科技项目(M-2022186)。

摘　　要：目的 研究中药提取物丹皮酚对大鼠卵母细胞体外培养体系氧化应激的改善作用,旨在确定丹皮酚改善氧化应激作用的最佳浓度,以期优化卵母细胞的体外培养体系,提高生产效率。方法 在卵母细胞的体外培养体系中分别添加2 mmol/L、4 mmol/L、6 mmol/L、8 mmol/L、10 mmol/L的丹皮酚,研究体外成熟后卵母细胞的成熟质量和氧化应激水平。结果 添加丹皮酚均能提高卵母细胞的成熟率(P<0.05)。添加8 mmol/L的丹皮酚获得卵母细胞的GVBD率、受精率和囊胚率高于其他组(P<0.05);添加6 mmol/L、8 mmol/L和10 mmol/L组的活性氧水平较低,与2 mmol/L、4 mmol/L组具有极显著差异(P <0.01)。添加丹皮酚能够显著提高成熟卵母细胞BCL-2基因和蛋白的表达水平,降低BAX基因和Caspase-3基因和蛋白的表达水平(P<0.01)。添加8 mmol/L、10 mmol/L组较其它组差异极显著(P<0.01)。结论 在大鼠卵母细胞体外成熟液中添加8 mmol/L的丹皮酚可以提高卵母细胞的体外成熟的生产效率和质量,且能够有效改善氧化应激水平、降低卵母细胞的凋亡。Objective To study the improvement effect of paeonol on oxidative stress in the rat oocytes in vitro maturation system,to determin the optimal concentration of paeonol for improving oxidative stress,to optimize the in vitro culture system and improve production efficiency.Methods 2mmol/L,4mmol/L,6mmol/L,8mmol/L and 10mmol/L of paeonol were added to the vitro maturation system to study the maturation quality and oxidative stress level of the rat oocytes in vitro maturation system.Results The addition of paeonol could significantly improve the maturation rate of oocytes compared to the control group(P<0.05).The GVBD rate,fertilization rate and blastocyst rate of oocytes in the 8 mmol/L paeonol group were higher than those of other experimental groups(P<0.05).The levels of reactive oxygen species in the 6 mmol/L,8 mmol/L and 10 mmol/L experimental groups were relatively low,and there were significant difference compared to the 2 mmol/L and 4 mmol/L experimental groups(P<0.01).Adding paeonol can significantly increase the expression levels of BCL-2 gene and protein in mature oocytes,and reduce the expression levels of BAX gene,Caspase-3 gene and protein(P<0.01).The groups of 8 mmol/L and 10 mmol/L showed significant differences compared to other groups(P<0.01).Conclusion Adding 8 mmol/L of paeonol to the in vitro maturation solution of rat oocytes could improve the production efficiency and quality of in vitro maturation of oocytes.It could also effectively improve the oxidative stress levels,and reduce the apoptosis of rat oocyte.

关 键 词：丹皮酚 大鼠 卵母细胞 体外成熟体系 氧化应激 

分 类 号：R285.6[医药卫生—中药学]