合欢皮总皂苷的提取工艺及其抗肿瘤活性研究  

The study on extraction process and anti-tumor activity of total saponins from albizia cortex

作　　者：仇亚茹 QIU Yaru(Heze Medical College,Heze 274000,Shandong)

机构地区：[1]菏泽医学专科学校,山东菏泽274000

出　　处：《菏泽医学专科学校学报》2024年第1期17-20,33,共5页Journal of Heze Medical College

基　　金：2022年山东省中医药科技项目(Q-2022094);菏泽医学专科学校博士启动基金项目。

摘　　要：目的 探讨合欢皮总皂苷的最佳提取工艺及其抗肿瘤活性。方法 以合欢皮为原料,应用乙醇回流技术提取合欢皮总皂苷,同时通过单因素实验研究乙醇浓度、提取时间、提取温度以及液料比等因素对合欢皮总皂苷提取率的影响。在此基础上,采用四因素三水平的正交实验,优化出最佳提取工艺。采用MTT法体外评价合欢皮总皂苷的抗肿瘤活性。结果 通过四因素三水平的正交实验,优化出合欢皮总皂苷的最佳提取工艺,即:乙醇浓度为70%,提取时间为1.5 h,提取温度为70℃,液料比为1:20(g/mL)。在此条件下,合欢皮总皂苷的提取率为31.20%。合欢皮总皂苷对人乳腺癌细胞(MDA-MB-231)的半抑制浓度(IC50值)为(9.92±0.83)μM。结论 实验结果表明合欢皮总皂苷对人乳腺癌细胞系(MDA-MB-231)具有一定的生物抑制活性。Objective To explore the optimum extraction conditions and anti-tumor activity of total saponins from albizia cortex.Methods Albizia cortex were used as raw material.Ethanol reflux technology was used to extract the total saponins of albizia cortex.Single-factor experiment was use to study the effect of ethanol concentration,extraction time,extraction temperature and solvent-to-solid ratio on the extraction efficiency.Based on this foundation,the orthogonal experiment of four factors and three levels was used to optimize the optimum extraction conditions.Furthermore,MTT assays was used to assess the antineoplastic activity of the total saponins from albiziae cortex in vitro.Results The optimum extraction conditions of total saponins from albizia cortex were obtained,ethanol concentration 70%,the extraction time 1.5 h,the extraction temperature 70℃,solvent-to-solid ratio 1:20(g/mL).Under these conditions,the extraction rate of total saponins from albiziae cortex was 31.20%.The IC50(μM)value of total saponins from albizia cortex against MDA-MB-231 was 9.92±0.83μM.Conclusion The results demonstrated that total saponins from albiziae cortex exhibited certain bioinhibitory activity against MDA-MB-231 cell lines.

关 键 词：合欢皮 总皂苷 提取工艺 乙醇 抗肿瘤 活性 

分 类 号：R283[医药卫生—中药学]