调控质粒拷贝数优化生物催化效率的研究进展  

Research progress on plasmid copy number regulation to optimize biocatalytic efficiency

作　　者：李业 严豪 刘梦婷 白仲虎 LI Ye;YAN Hao;LIU Mengting;BAI Zhonghu(School of Biotechnology,Jiangnan University,Wuxi 214122,China;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China;Institute of Biopharmaceutical and Health Engineering,Shenzhen International Graduate School,Tsinghua University,Shenzhen 518000,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学,粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]清华大学,深圳国际研究生院生物医药与健康工程研究院,广东深圳518000

出　　处：《食品与发酵工业》2024年第6期282-289,共8页Food and Fermentation Industries

基　　金：国家自然科学基金面上项目(21878142);江南大学青年基金(JUSPR12057)。

摘　　要：质粒作为外源基因异源表达的普遍载体,在合成生物学中广泛应用。不同质粒载体在细胞工厂中的拷贝数不尽相同,合理利用拷贝数不同的质粒可以优化目标基因表达水平,提高生物催化效率。针对天然质粒复制子系统的局限,近年来研究者们依据质粒复制机制,开发出了许多性能更优异的新质粒系统,显著提高了生物催化效率。该文结合质粒拷贝数在多种微生物细胞工厂中的应用进行综述,以期为合成生物学领域的相关研究提供参考和借鉴。As a universal vector for heterologous expression of foreign genes,plasmids are widely used in synthetic biology.Different plasmid vectors have different copy numbers in cell factories.Rational use of plasmids with different copy numbers can optimize the expression level of target genes and improve biocatalytic efficiency.In response to the limitations of natural plasmid replicon systems,in recent years researchers have developed many new plasmid systems with better performance based on plasmid replication and copy number control mechanisms,significantly improving biocatalytic efficiency.This article reviewed plasmid systems in various microbial cell factories along with plasmid copy number engineering and applications in optimizing biocatalytic efficiency,providing a reference for related research in the field of synthetic biology.

关 键 词：异源表达 质粒 复制子 拷贝数 合成生物学 

分 类 号：Q78[生物学—分子生物学] Q81