微小核糖核酸-451a在人参养荣汤治疗鼻咽癌放射性黏膜损伤中的机制研究  

Study on the mechanism of miR-451a in the treatment of radiation induced mucosal damage in nasopharyngeal cancer with ginseng yangrong tang

作　　者：鲁志兵[1] 李瑞娟 吴志清[1] 肖永娟[1] LU Zhibing;LI Ruijuan;WU Zhiqing;XIAO Yongjuan(Pingxiang People's Hospital,Pingxiang 337000,China)

机构地区：[1]萍乡市人民医院,江西萍乡337000

出　　处：《临床医药实践》2024年第4期243-247,共5页Proceeding of Clinical Medicine

基　　金：江西省中医药管理局科技计划项目(项目编号:2022A124)。

摘　　要：目的:通过检测微小核糖核酸-451a(miR-451a)和富含半胱氨酸的分泌性酸性蛋白(SPARC)mRNA的表达,推断人参养荣汤治疗鼻咽癌(NPC)放射性黏膜损伤的疗效及作用机制。方法:选择2022年3月—2023年5月收治的鼻咽癌放射性口腔黏膜损伤患者30例作为观察组,选择同期健康者30例作为对照组。采用辨证论治拟人参养荣汤加减治疗,比较观察组治疗前后患者中医症候积分量表和口干程度、口咽疼痛程度、黏膜损伤程度。检测治疗前后患者血清miR-451a和SPARC mRNA表达水平。结果:人参养荣汤可以有效降低患者主症和次症严重程度、Ⅲ级和Ⅳ级口腔黏膜损伤程度、口咽疼痛程度以及口干程度,使患者血清中miR-451a表达下降,促进SPARC mRNA蛋白翻译增强(P<0.05)。治疗前后,miR-451a与中医症候积分均呈正相关(P<0.05)。结论:人参养荣汤对鼻咽癌放射性黏膜损伤具有一定的临床疗效,可降低miR-451a表达水平,升高SPARC mRNA表达水平;miR-451a抑制了SPARC mRNA的翻译,可为将来的中西医结合治疗提供理论依据。Objective:To observe the therapeutic effect of ginseng yangrong tang on radiation induced mucosal damage in nasopharyngeal carcinoma,and to infer the mechanism of action by detecting the expression of micro RNA-451a(miR-451a)and secreted protein acidic and rich in cysteine(SPARC)mRNA.Methods:Thirty patients with radiation-induced oral mucosa injury of nasopharyngeal carcinoma were selected from March 2022 to May 2022 as the observation group and 30 healthy 2023 were selected as the control group.The treatment group was treated with modified shenyangrong decoction based on syndrome differentiation,and the traditional chinese medicine symptom integral scale,the degree of xerostomia,the degree of oropharyngeal pain and the degree of mucosa injury were compared before and after treatment.The levels of Mir-451a and SPARCmRNA were measured before and after treatment.Results:Ginseng yangrong Tang can effectively reduce the severity of the main and secondary symptoms,the degree of oral mucosa injury of gradeⅢandⅣ,the degree of oropharyngeal pain and the degree of xerostomia,and decrease the expression of miR-451a in the serum of the patients,sPARC mRNA translation was enhanced(P<0.05).There was a positive correlation between MIR-451 a and TCM symptom scores before and after treatment(P<0.05).Conclusion:Ginseng yangrong tang has a certain clinical therapeutic effect on radiation induced mucosal damage in nasopharyngeal carcinoma.Ginseng yangrong tang can reduce the expression level of miR-451a and increase the expression level of SPARC mRNA,while miR-451a inhibits the translation of SPARC mRNA,providing a theoretical basis for future integrated chinese and western medicine treatment.

关 键 词：微小核糖核酸-451a 人参养荣汤 鼻咽癌放射性黏膜损伤 富含半胱氨酸的分泌性酸性蛋白 机制研究 

分 类 号：R273[医药卫生—中西医结合]