决明Aux/IAA和ARF基因家族鉴定、表达及蛋白互作分析  

Identification,expression and protein interaction analysis of Aux/IAA and ARF gene family in Senna tora L.

作　　者：冯昭 刘世鹏 吕蕊花 吕瑞华 胡晓晨 张明英 毛仁俊 张岗[2] FENG Zhao;LIU Shi-peng;LÜRui-hua;LÜRui-hua;HU Xiao-chen;ZHANG Ming-ying;MAO Ren-jun;ZHANG Gang(College of Medical Technology,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Key Laboratory for Research of"Qin Medicine"of Shaanxi Administration of Chinese Medicine,College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;College of Life Science,Yan'an University,Yan'an 716000,China)

机构地区：[1]陕西中医药大学医学技术学院,陕西咸阳712046 [2]陕西中医药大学药学院,陕西省中医药管理局“秦药”研发重点实验室,陕西咸阳712046 [3]延安大学生命科学学院,陕西延安716000

出　　处：《药学学报》2024年第3期751-763,共13页Acta Pharmaceutica Sinica

基　　金：陕西省教育厅专项科研计划项目(23JK0401,21JK0989);陕西中医药大学校级科研课题(2021GP07);陕西省自然科学基金(2021JQ-636);陕西中医药大学学科创新团队(2019-QN01).

摘　　要：植物生长素早期响应基因家族(auxin/indole-3-acetic acid,Aux/IAA)及其与生长素响应转录因子(auxin response factor,ARF)的互作是调控植物生长发育的重要方式。本文基于药用植物决明(Senna tora L.)全基因组数据鉴定到28个StoIAA和24个StoARF成员,分别归为10个和8个亚家族。系统发育树和共线性分析发现,决明与大豆(Glycine max)、蒺藜苜蓿(Medicago truncatula)的IAA、ARF同源基因均存在密切的进化关系,且基因片段重复事件主导了StoIAA和StoARF的扩张。基因结构分析显示,绝大多数StoIAAs和StoARFs含有特征保守结构域。转录组数据分析显示,StoIAAs和StoARFs在叶、根和种子中均有表达,部分成员具有组织表达特异性。StoIAA和StoARF启动子区域多含有与胁迫响应、生长发育、激素诱导、次生代谢等相关的作用元件。基因表达分析显示许多StoIAAs和StoARFs能够迅速响应干旱和盐胁迫,且在两种胁迫条件下表现出相似的表达模式。酵母双杂交实验证实StoARF8和StoARF10分别与多个StoIAA蛋白之间同时存在不同程度的互作。以上研究结果为进一步开展决明Aux/IAA和ARF基因家族的生物学功能分析提供基础。The early response of plant auxin gene family Aux/IAA(auxin/indole-3-acetic acid)and its interaction with auxin response factor(ARF)are important pattern to regulate plant growth and development.This work identified 28 StoIAA and 24 StoARF members based on the whole genome data of the medicinal plant Senna tora L.,which were classified into 10 and 8 subfamilies,respectively.Phylogenetic tree and collinearity analysis showed that S.tora has close evolutionary relationship with the IAA and ARF homologous genes of Glycine max,Medicago truncatula,and the segment duplication events dominate the expansion of StoIAA and StoARF.Gene structure analysis showed that the vast majority of StoIAA and StoARF contain characteristic conserved domain.Transcriptome data showed that StoIAAs and StoARFs were expressed in leaves,roots and seeds,some members had tissue specific expression.The StoIAA and StoARF promoter region most contain functional elements related to stress response,growth and development,hormone induction and secondary metabolism.In addition,gene expression analysis showed that many StoIAAs and StoARFs can quickly respond to drought and salt stress and exhibited same expression patterns under both stress condition.The yeast two-hybrid experiment confirmed that StoARF8 and StoARF10 exhibit varying degrees of interaction with multiple StoIAA proteins,respectively.The above results provide a basis for further biological functional analysis of the Aux/IAA and ARF gene family of S.tora.

关 键 词：决明 AUX/IAA 生长素响应转录因子 激素 蛋白互作 

分 类 号：R931[医药卫生—生药学]