77份山西黍稷DNA二维码身份证的构建  

Construction of 77 Two-Dimensional DNA Identity Cards of Shanxi Broomcorn Millet

作　　者：曹越 曹晓宁 陈凌[2] Santra Dipak K 乔治军 王瑞云[1,2] CAO Yue;CAO Xiaoning;CHEN ling;SANTRA Dipak K;QIAO Zhijun;WANG Ruiyun(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030031,China;Panhandle Research and Extension Center,Department of Agronomy and Horticulture,University of Nebraska Lincoln,Scottsbluff 69361,USA)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学农业基因资源研究中心/农业部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室,山西太原030031 [3]内布拉斯加大学林肯分校农艺系小宗粮豆研究与推广中心,美国内布拉斯加州69361

出　　处：《山西农业科学》2024年第2期8-15,共8页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31271791);国家现代农业产业技术体系建设专项(CARS-06-14.5-A16);山西省现代农业产业技术体系建设(杂粮)项目(2022-03);山西省重点研发项目(2022ZDYF110)。

摘　　要：为了更好地管理黍稷资源,分辨其身份以及追溯来源,创建一个高效可行的种质资源鉴定系统十分必要。以山西省内各地77份黍稷资源为材料,用上海生工生物股份有限公司合成上游5’端加FAM (blue)荧光基团标记对77份黍稷资源进行PCR及毛细管电泳。结果发现,仅用5个标记组合(RYW3、RYW6、RYW20、RYW37和RYW40)可区分全部材料。其中,组合RYW6+RYW37可区分60份;组合RYW6+RYW20+RYW37可区分69份;组合RYW6+RYW20+RYW37+RYW40可区分73份;组合RYW3+RYW6+RYW20+RYW37+RYW40可区分全部77份。77份材料在8个位点共检出79个等位变异,每个位点检出平均为9.875个,检测到的有效等位变异(Ne)为2.675 9,Shannon多样性指数(I)为0.991 6,Nei’s基因多样性指数(Nei)为0.426 0,多样性信息含量(PIC)为0.553 3。在此基础上,利用ID analysis 4.0在线条形码生成器将对应字符串生成可扫描的条形码DNA分子身份证;利用二维码在线技术将材料基本信息转化成可扫描的二维码DNA分子身份证。In order to better manage broomcorn millet resources,distinguish their identities and trace their sources,it is necessary to create an efficient and feasible germplasm resource identification system.In this study,77 broomcorn millet resources in Shanxi Province were used as the materials.PCR and capillary electrophoresis were carried out on 77 broomcorn millet resources with FAM(blue)fluorescent group labeling at the upstream 5'end synthesized by Shanghai Sangon Biological Co.,Ltd.It was found that only five marker combinations(RYW3,RYW6,RYW20,RYW37,and RYW40)could distinguish all materials.Among them,the combination of RYW6+RYW37 could distinguish 60 materials,the combination of RYW6+RYW20+RYW37 could distinguish 69 materials,the combination of RYW6+RYW20+RYW37+RYW40 could distinguish 73 materials,and the combination of RYW3+RYW6+RYW20+RYW37+RYW40 could distinguish all 77 materials.A total of 79 alleles were detected in 77 materials at 8 loci,with an average of 9.875 detected at each locus.The effective allele variation(Ne)detected was 2.6759,Shannon diversity index(I)was 0.9916,Nei's gene diversity index(Nei)was 0.4260,and polymorphism information content(PIC)was 0.5533.On this basis,the ID analysis 4.0 online barcode generator was used to generate a scannable barcode DNA molecular identity cards.Using two-dimensional code online technology,the basic information of the materials was transformed into scannable two-dimensional code DNA molecular identity cards.

关 键 词：SSR 黍稷 二维码 山西 

分 类 号：S516[农业科学—作物学]