抗感菜豆品种对菜豆普通花叶病毒侵染后的转录组分析  

Transcriptome Analysis of Resistant and Susceptible Common Bean Varieties Infected by Bean Common Mosaic Virus

作　　者：牛静雅 唐慕宁 王新华 霍思凡 武文艳 梁兴瑞 黄欣琪 王古悦 冯雪 NIU Jingya;TANG Muning;WANG Xinhua;HUO Sifan;WU Wenyan;LIANG Xingrui;HUANG Xinqi;WANG Guyue;FENG Xue(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学植物保护学院,山西太谷030801

出　　处：《山西农业科学》2024年第2期65-77,共13页Journal of Shanxi Agricultural Sciences

基　　金：山西省回国留学人员科研资助项目(2020-063);国家自然科学基金项目(32202247);山西省自然科学基金项目(20210302123367)。

摘　　要：菜豆普通花叶病毒(BCMV)在世界范围内分布广泛并可造成菜豆严重减产。菜豆是BCMV的主要寄主,不同遗传背景的菜豆对BCMV侵染的响应存在显著差异,目前菜豆抗性基因功能及BCMV的致病机制鲜有报道。为了为菜豆抗性基因功能的研究以及分子抗病育种提供相关依据,利用转录组测序(RNA sequencing)技术对BCMV(C54株系)侵染感性及不同抗性的菜豆品种材料进行转录组测序,对所得数据筛选差异表达基因并进行Gene Ontology(GO)、Kyoto Encyclopedia of Genes and Genomes(KEGG)富集以及进行Weighted correla?tion network analysis(WGCNA)分析。结果发现,不同的菜豆品种中病毒的积累量、差异表达基因的定位及一些关键通路对病毒侵染的响应存在共性和差异(如昼夜节律、光合作用、植物-病原体的相互作用和一些代谢途径相关通路等)。在接种叶上,Dubbele witte品种(DW,对BCMV侵染易感)与Redland’s greenleaf C品种(RGLC,对BCMV侵染具有抗性)差异表达的基因类型上更为相似,定位于光合体系,在光合作用、光合作用-天线蛋白以及光合生物中的碳固定等通路富集,而Sanilac(对BCMV侵染具有抗性)的差异基因没有在光合作用等通路富集。在系统叶上,2个抗性品种中的差异表达基因类型也有差异。植物被病毒侵染后会干扰植物激素的合成与信号转导通路。通过选取8个关键的植物激素合成或信号转导相关基因进行验证,转录组和qPCR数据结果表明,不同的植物激素合成或信号转导相关基因在BCMV侵染菜豆后的表达情况存在差异:BCMV侵染促进了参与水杨酸、茉莉酸和赤霉素合成通路的关键基因的正向调控;乙烯、油菜素内酯以及脱落酸相关的基因在抗感品种中的表达变化趋势不相同。研究结果明确了BCMV侵染不同遗传背景的菜豆品种后的转录组差异反应。Bean Common Mosaic Virus(BCMV)is widely distributed worldwide and can cause a serious reduction in common bean yields.Common bean is the primary host of BCMV,and there are significant differences in the response of common beans with different genetic backgrounds to BCMV infection.Currently,there are few reports on the function of resistant genes in common beans and the pathogenic mechanism of BCMV.To provide a data foundation for the study on resistant gene function and molecular disease-resistance breeding for common bean varieties,in this study,RNA-sequencing technology was utilized to conduct transcriptional sequencing of common bean varieties with varying levels of susceptibility to BCMV infection(C54 strain).The obtained data were analyzed to identify differentially expressed genes,and further analyses were conducted using gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG)enrichment,and weighted correlation network analysis(WGCNA).It was observed that there were both commonalities and differences in virus accumulation,mapping of differentially expressed genes,and responses of key pathways to virus infection(such as circadian rhythm,photosynthesis,plant-pathogen interaction,and various metabolic pathways)in different common bean varieties.In the infected leaves,the types of differentially expressed genes in Dubbele witte variety(DW,susceptible)and Redland’s greenleaf C variety(RGLC,resistant)were more similar to each other,mostly localized in photosynthetic systems and enriched in pathways related to photosynthesis,photosynthesis-antenna protein,and carbon fixation in photosynthetic organisms.Conversely,Sanilac variety(resistant)differential genes did not exhibit enrichment in photosynthesis or other pathways.Additionally,in systemic leaves,the types of differentially expressed genes were distinct in the two resistant varieties.Plant infection by the virus can disrupt plant hormone synthesis and signal transduction pathways.Eight key genes involved in plant hormone synthesis or signal transduct

关 键 词：菜豆普通花叶病毒 转录组 WGCNA 菜豆 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]