小片蝽线粒体基因组及蝽科系统发育分析  

Mitochondrial Genome of Sciocoris lateralis and Phylogenetic Analysis of Pentatomidae

作　　者：刘迎香 赵清 LIU Yingxiang;ZHAO Qing(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学植物保护学院,山西太谷030801

出　　处：《山西农业科学》2024年第2期78-88,共11页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31872272);山西省回国留学人员科研资助项目(2020-064,2020-065)。

摘　　要：为了从线粒体基因组水平探讨小片蝽Sciocoris lateralis在蝽科的分类地位,丰富蝽科线粒体基因组基本数据,为蝽科系统发育进化研究提供一定的理论依据,通过高通量测序,首次测定并分析了小片蝽完整线粒体基因组(Genbank登录号:OP531920),提取蝽科物种的13个蛋白编码串联序列(PCGs),使用最大似然法、贝叶斯法构建系统发育树。结果显示,小片蝽昆虫的线粒体全基因组长度为15 445 bp,包括13个蛋白编码基因(Protein-codinggenes,PCGs)、2个rRNA基因(ribosomeRNAs,rRNAs)、22个tRNA基因(transferRNAs,tRNAs)和1个控制区(Control region)。小片蝽线粒体基因组结构排序与其他蝽科物种一致,均无基因重排现象。小片蝽线粒体全序列AT含量为74.26%,GC含量为25.74%。13个蛋白编码基因中,cox1、nad1和nad6的起始密码子为TTG,其余10个蛋白编码基因的起始密码子是ATT、ATA、ATG。在所测得的tRNA基因中,trnS1和trnV缺失DHU臂,其他20个tRNA均能折叠形成典型的三叶草结构。基因错配方式主要为G-U错配。基于13个PCGs构建的蝽科系统发育树结果显示,小片蝽与斑须蝽的亲缘关系最近并且位于蝽亚科分支,支持二星蝽族Eysarcorini和Strachiini族的单系性,而棕蝽族Caystrini和腹沟族Halyini互为姐妹群,这与传统形态学分类结果一致;益蝽亚科与蝽亚科曼蝽属(Menida)形成姐妹群关系,该系统发育关系与传统形态分类学研究结果不一致。In order to explore the taxonomic position of Sciocoris lateralis in the family Pentatomidae at the mitochondrial genome level,enrich the basic mitochondrial genome data of the family Pentatomidae,and provide a theoretical basis for the phylogenetic evolution of Pentatominae,in this study,the complete mitochondrial genome of S.lateralis was determined and analyzed for the first time through high-throughput sequencing(Genbank login Number:OP531920).13 protein-coding tandem sequences(PCGs)from species of Pentatomidae were extracted and phylogenetic trees were constructed using the maximum likelihood method and Bayesian method.The results showed that the mitochondrial genome length of the insects was 15445 bp,including 13 Protein-coding genes(PCGs),2 rRNA genes(ribosomal RNAs),22 tRNA genes(transfer RNAs),and a control region.The sequence of the mitochondrial genome was consistent with that of other species,and there was no gene rearrangement.The content of AT and GC was 74.26%and 25.74%,respectively.Among the 13 protein-coding genes,the start codons of cox1,nad1,and nad6 were TTG,while the start codons of the other 10 protein-coding genes were ATT,ATA,and ATG.Among the measured tRNA genes,trnS1 and trnV were missing DHU arms,while the other 20 tRNAs were able to fold to form a typical clover structure.The main pattern of gene mismatch was G-U mismatch.Based on the 13 PCGs,the phylogenetic tree of Pentatomidae showed that S.lateralis and Dolycoris baccarum were closely related and located in the subfamily of Pentatominae,supporting the monophyletic families of Eysarcorini and Strachiini,while Caystrini and Halyini were sister groups.This was consistent with the traditional morphological classification.Asopinae and Menida were sister groups,the phylogenetic relationship was inconsistent with the conclusion of traditional morphological taxonomy.

关 键 词：小片蝽 线粒体基因组 蝽科 系统发育 

分 类 号：Q969.351.6[生物学—昆虫学]