莲草直胸跳甲3个气味结合蛋白基因的克隆与表达分析  

Cloning and Expression Analysis of three Odorant Binding Protein Genes in Agasicles hygrophila

作　　者：皇甫鸿雁 付淑慧 贾栋[1] 王苑馨 胡军 马瑞燕[1] HUANGFU Hongyan;FU Shuhui;JIA Dong;WANG Yuanxin;HU Jun;MA Ruiyan(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学植物保护学院,山西太谷030801

出　　处：《山西农业科学》2024年第2期89-96,共8页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31570436);山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202003-4)。

摘　　要：气味结合蛋白昆虫嗅觉通讯过程中的重要蛋白质,参与昆虫气味识别的第一步反应,在昆虫觅食、聚集、求偶和寻找产卵场所等活动中起重要作用。为明确莲草直胸跳甲(Agasicles hygrophila)气味结合蛋白基因(OBP)表达特征,初步了解其功能,对3个OBP基因进行了克隆与生物信息学分析,并通过实时荧光定量PCR分析了它们在不同组织中的相对表达量。结果显示,AhygOBP27、AhygOBP32与AhygOBP44的开放阅读框分别为351、408、423 bp,其编码的蛋白质是分子量较小的酸性可溶性蛋白质。其中,AhygOBP27主要在雌雄虫的触角、头、足、翅中表达;AhygOBP32主要在雌雄虫的触角、足、翅以及雄虫的头中表达;AhygOBP44主要在雌雄虫的触角、头、足中表达。这3个基因在雌雄成虫触角中的表达水平均显著高于其他组织,其中AhygOBP27与AhygOBP44在雌性触角中的表达水平显著高于雄性;AhygOBP32在雄性触角中的表达水平显著高于雌性。推测这3个基因在莲草直胸跳甲嗅觉识别过程中起重要作用,AhygOBP27和AhygOBP44可能与识别寄主植物挥发物和寻找产卵地相关,AhygOBP32可能参与雄虫识别雌虫释放的性信息素。Odorant binding protein(OBP)is an important protein in the process of insect olfactory communication.OBPs are involved in the first step of insect odor recognition,and play an important role in insect activities such as foraging,aggregation,courtship,and looking for spawning places.In order to determine the expression characteristics of OBP genes in Agasicles hygrophila and to preliminarily understand its functions,in this study,three OBP genes were cloned and analyzed using bioinformatics.The relative expression levels of OBPs in different tissues were analyzed by quantitative real-time PCR.The results showed that the open reading frame of AhygOBP27,AhygOBP32,and AhygOBP44 were 351,408,423 bp,respectively.The proteins encoded by the three OBPs were acidic soluble proteins with small molecular weight.AhygOBP27 was mainly expressed in the antennae,head,feet and wings of both sexes.AhygOBP32 was expressed in antennae,head,leg and wings of the male and in antennae,feet and wings of the female.AhygOBP44 was expressed in the antennae,heads and feet of both sexes.The expression levels of above three genes in the antennae of male and female were significantly higher than that of other tissues.The expression level of AhygOBP27 and AhygOBP44 in female antennae were significantly higher than that in male antennae,and the expression level of AhygOBP32 in the antennae of males was significantly higher than that of females.The three genes might play an important role in the olfactory recognition of Agasicles hygrophila,AhypOBP27,and AhygOBP44 might be related to the recognition of host plant volatiles and the search for spawning ground;AhygOBP32 might be involved in the recognition of sex pheromone emitted by females.

关 键 词：喜旱莲子草 莲草直胸跳甲 气味结合蛋白 基因克隆 组织表达 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]