金茵清热口服液通过NF-κB/NLRP3信号通路改善小鼠急性肺损伤  

Jinyinqingre Oral Liquid Alleviates Acute Lung Injury in Mice through NF-κB/NLRP3 Signaling Pathway

作　　者：王淑惠 李小兵 沈婷 雷攀 刘泽干 杜士明 WANG Shuhui;LI Xiaobing;SHEN Ting;LEI Pan;LIU Zegan;DU Shiming(School of Pharmacy,Hubei University of Medicine,Shiyan 442000,China;Department of Respiratory and Critical Care Medicine,Taihe Hospital,Affiliated to Hubei University of Medicine,Shiyan 442000,China;School of Pharmacy,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China;Department of Scientific Research,Taihe Hospital Affiliated to Hubei University of Medicine,Shiyan 442000,China;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院药学院,十堰442000 [2]湖北医药学院附属太和医院呼吸与危重症医学科,十堰442000 [3]湖北中医药大学药学院,武汉430065 [4]湖北医药学院附属太和医院科研处,十堰442000 [5]武当特色中药研究湖北省重点实验室·湖北医药学院,十堰442000

出　　处：《医药导报》2024年第4期520-525,共6页Herald of Medicine

基　　金：湖北医药学院研究生科技创新项目(YC2022022);湖北省卫生健康委员会资助项目(ZY2021M005);湖北省科技厅项目(2021CFB247);湖北省教育厅科学技术研究项目(B2019110)。

摘　　要：目的探究金茵清热口服液对脂多糖(LPS)诱导小鼠急性肺损伤的保护作用及机制。方法C57BL/6J小鼠按随机数字表法分为6组:空白对照组、模型对照组、金茵清热口服液小剂量组、金茵清热口服液中剂量组、金茵清热口服液大剂量组和地塞米松组。除空白对照组,其他各组气管滴注LPS溶液(5 mg·kg^(-1))构建小鼠急性肺损伤模型,金茵清热口服液低中高组连续灌胃给药3 d。24 h后,6组分别取小鼠肺组织和支气管肺泡灌洗液(bronchoalveolar larage fluid,BALF)进行后续检测,HE染色观察各组小鼠肺组织病理损伤;检测肺泡灌洗液总细胞数;BCA法检测BALF的总蛋白含量;ELISA法检测BALF中炎性细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和免疫球蛋白M(IgM)的含量;免疫组化和Western blotting法检测肺组织核因子κB(NF-κB)、NOD样受体热蛋白结构域相关蛋白3(NLRP3)蛋白的表达情况。结果与模型对照组比较,金茵清热口服液减轻了肺组织的病理学损伤(P<0.05),降低了肺泡灌洗液中总细胞数、总蛋白含量和IgM的表达(P<0.05),肺泡灌洗液中炎性细胞因子的TNF-α、IL-6和IL-1β的表达(P<0.01),肺组织NF-κB、NLRP3蛋白表达(P<0.05)。结论金茵清热口服液减轻了LPS诱导小鼠肺损伤的病理损伤,降低了炎性渗出和炎性细胞因子的表达,其作用机制可能是通过调控NF-κB/NLRP3信号通路,为其临床应用提供理论依据。Objective To explore the protective effect and mechanism of Jinyinqingre oral liquid on acute lung injury induced by lipopolysaccharide(LPS)in mice.Methods C57BL/6J mice were randomly divided into six groups according to the random number table method:blank control group,model control group,Jinyinqingre oral liquid low-dose group,Jinyinqingre oral liquid medium-dose group,Jinyinqingre oral liquid high-dose group,and dexamethasone group.Except for the blank control group,the other groups were injected with lipopolysac charide(LPS)(5 mg·kg^(-1))into the trachea to establish the acute lung injury model of mice,and the Jinyinqingre oral liquid low,medium,and high groups were continuously administered the drug by gavage for three days.After 24 h,lung tissue and bronchoalveolar lavage fluid(BALF)were collected from the six groups for follow-up detection.The pathological injury of lung tissue in each group was observed by HE staining.The total number of cells in BALF was detected.The total protein content of BALF was detected by the BCA method.The contents of inflammatory cytokines TNF-α,IL-1β,IL-6,and IgM in BALF were detected by ELISA.The expression of NF-κB and NLRP3 proteins in lung tissues was detected by immunohistochemistry and Western blotting.Results Compared with model control group,Jinyinqingre oral liquid alleviated the pathological injury of lung tissue(P<0.05),decreased the total cell count,total protein content and IgM expression in BALF(P<0.01),and the expression of inflammatory cytokines TNF-α,IL-6 and IL-1βin BALF was dreased(P<0.05),the protein expressions of NF-κB and NLRP3 in lung tissues was dreased(P<0.05).Conclusion Jinyinqingre oral liquid attenuated the pathological injury,inflammatory exudation,and expression of inflammatory cytokines in LPS-induced lung injury in mice,and its mechanism may be through the regulation of NF-κB/NLRP3 signaling pathway,providing a theoretical basis for its clinical application.

关 键 词：金茵清热口服液 脂多糖 急性肺损伤 NF-κB/NLRP3信号通路 炎症反应 

分 类 号：R97[医药卫生—药品] R563[医药卫生—药学]