高效液相色谱法同时测定维药香青兰有效部位中10个化学成分的含量  

Simultaneous Determination of 10 Effective Components in the Active Parts of Uygur Medicine Dracocephalum Moldavica L.by HPLC

作　　者：谭梅娥 何承辉 帕依曼·亥米提 郑瑞芳 于宁 邢建国 TAN Mei'e;HE Chenghui;Payman·Hermity;ZHENG Ruifang;YU Ning;XING Jianguo(Xinjiang Uyghur Pharmaceutical Laboratory,Xinjiang Uygur Autonomous Region Institute of Meteria Medica,Urumqi 830011,China)

机构地区：[1]新疆维吾尔自治区药物研究所新疆维吾尔药实验室,乌鲁木齐830011

出　　处：《医药导报》2024年第4期567-571,共5页Herald of Medicine

基　　金：中央引导地方科技发展专项资助项目(ZYYD2022A02);国家自然科学基金资助项目(81960788);新疆维吾尔自治区自然科学基金青年基金项目(2019D01B45);新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2019101,KY2020087)。

摘　　要：目的建立高效液相色谱(HPLC)法同时测定维药香青兰有效部位中10个成分含量。方法采用Shim-pack ODS(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈(A)-0.5%甲酸溶液(B),梯度洗脱(0—30 min,17%A;30—60 min,17%→28%A;60—78 min,28%A),流速为1.0 mL·min-1;检测波长为330 nm,柱温为35℃。结果咖啡酸、4-香豆酸、木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-葡萄糖醛酸苷、迷迭香酸、香叶木素-7-O-β-D-葡萄糖醛酸苷、丹酚酸A、田蓟苷、香叶木素在该色谱条件下分离度良好,在考察的浓度范围内线性关系良好(r>0.9992)。加样回收率和相对标准偏差(RSD)分别为91.83%~106.43%和0.38%~2.22%内。结论建立的含量测定方法准确性高、重复性好,适用于香青兰有效部位的分析与质量控制研究。Objective To establish an HPLC method for the simultaneous determination of 10 components in the active parts of Uygur medicine Dracocephalum Moldavica L.Methods The determination was performed on a Shim-pack ODS(4.6 mm×250 mm,5 um)column with the mobile phase consisting of acetonitrile(A)-0.5%formic acid(B)in aqueous solution in a gradient elution mode(0-30 min,17%A;30-60 min,17%→28%A;60-78 min,28%A)at a flow rate of 1.0 mL·min-1.The temperature of the chromatographic column was 35℃and the detection was monitored by a UV detector at 330 nm.Results Coffeic acid,p-coumalic acid,cynaroside,luteolin-7-O-β-D-glucuronide,apigenin 7-O-glucuronide,rosmarinic acid,diosmetin7-O-β-D-glucuronide,salvianolic acid A,tilianin,apigenin were well separated under this chromatographic condition,and the linear relationship were good in the concentration range examined(r>0.9992).The overall recoveries ranged from 91.83%to 106.43%with the RSD ranging from 0.38%to 2.22%.Conclusion The established content determination method is highly accurate and reproducible,and suitable for the analysis and quality control of the active parts of Dracocephalum Moldavica L.

关 键 词：香青兰有效部位 香叶木素-7-O-β-D-葡萄糖醛酸苷 田蓟苷 高效液相色谱法 

分 类 号：R97[医药卫生—药品] R284[医药卫生—药学]