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作 者:陈欢 张健 黄恒成 唐正涛 韦联健 周韬 邓紫宇[2] CHEN Huan;ZHANG Jian;HUANG Hengcheng;TA NG Zhengtao;WEI lianjian;ZHOU Tao;Deng Ziyu(Guangxi Forestry Group Guiqin Forestry&Pulp&Paper Co.,Ltd.,Nanning 530022,Guangxi,China;Guangxi Forestry Research Institute,Guangxi Nanning Eucalypt Plantation E cosystem Observation and Research Station,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning 530002,Guangxi,China;College of Forestry,Hainan University,Haikou 570228,Hainan,China)
机构地区:[1]广西林业集团有限公司桂钦林浆纸有限公司,广西南宁530022 [2]广西壮族自治区林业科学研究院、广西南宁桉树森林生态系统定位观测研究站广西优良用材林资源培育重点实验室,广西南宁530002 [3]海南大学林学院,海南海口570228
出 处:《福建林业科技》2024年第1期1-8,共8页Journal of Fujian Forestry Science and Technology
基 金:广西林业科技推广示范项目(桂林科研[2022]第10号);海南省重点研发项目(ZDYF2020046)。
摘 要:2021年对广西六峰山林场的大叶桉(Eucalyptus robusta)进行采样,采用Hiseq 2000对大叶桉根、茎、叶组织分别进行转录组测序。结果获得40786个Unigene;通过数据库比对,共注释了31662个Unigene。GO注释结果最多的条目为膜组成部分;KEGG注释结果表明,5654条序列参与129条代谢通路,参与基因最多的通路为核糖体。对比分析大叶桉根部和茎,以及根部和叶之间的转录差异,发现多条富集的通路,包括α-亚麻酸代谢和萜类骨架生物合成等。鉴定出AP 2/ERF家族基因84个,其中AP 2家族含有9个基因,RAV家族含有2个基因,ERF家族包含73个基因,各基因家族在根茎叶中的表达模式有所差异。In 2021,Eucalyptus robusta was sampled from Liufengshan forestr y center in Guangxi Province.Transcriptome sequencing was performed on the root,stem and leaf tissues of E.robusta by Hiseq2000.40786 unigen es were obtained.A total of 31662 unigenes were annotated by database alignment.The items with the most GO annotation results were membrane components.The KEGG annotation re sults showed that 5654 sequences participated in 129 metabolic pathways,and the pathway with the most genes was ribosome.A comparative analysis of the transcr iptional differences between the roots and stems of E.robusta,as well as betwe en the roots and leaves,revealed multiple enriched pathways,includingα-Lin o lenic acid metabolism and terpene backbone biosynthesis.84 AP 2/ERF family gene s were identified,including 9 genes in AP 2 family,2 genes in RAV family and 73 genes in ERF family.Each gene family has different expression patterns in root,stem and leaf.
分 类 号:S792.39[农业科学—林木遗传育种]
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