湖南某规模奶牛场乳房炎病原菌的分离鉴定与耐药性分析  

Isolation,Identification and Drug Resistance Analysis of Mastitis Pathogenic Bacteria froma Large-scale Dairy Farm in Hunan Province

作　　者：刘伯承[1] 史芳 杨俊[1] 周望平[1] LIU Bo-cheng;SHI Fang;YANG Jun;ZHOU Wang-ping(Hunan Institute of Animal and Veterinary Science,Changsha 410131;Agricultural and Rural Office of Intersection Town,Changsha 410142)

机构地区：[1]湖南省畜牧兽医研究所,长沙410131 [2]湖南省长沙县路口镇农业农村办,长沙410142

出　　处：《中国奶牛》2024年第3期29-33,共5页China Dairy Cattle

基　　金：“十三五”国家重点研发计划(2018YFD050170);长沙市重点科技计划项目(kh20201218)。

摘　　要：为明确湖南某规模奶牛场临床型乳房炎病原菌种类及耐药情况,对32份临床型乳房炎患牛乳样进行传统细菌培养、生化试验、16S rDNA核苷酸序列检测和药敏试验。结果共分离鉴定大肠杆菌23株,乳房链球菌12株,肺炎克雷伯氏菌5株,样品检出率分别为71.9%(23/32)、37.5%(12/32)、15.6%(5/32);药敏试验显示,大部分大肠杆菌和乳房链球菌对青霉素、阿莫西林、头孢他啶、庆大霉素、林可霉素耐药严重,耐药率达50%~86.9%,对亚胺培南、四环素及红霉素较敏感,敏感率达75%~82.6%;肺炎克雷伯氏菌对大部分抗生素耐药程度较低,对亚胺培南、阿米卡星、四环素、红霉素敏感率为100%。建议该养殖场抓好环境卫生、提高生物安全,建立科学、合理治疗方案。To ascertain the pathogenic species and drug resistance of clinical dairy mastitis in a large-scale dairy farm in Hunan,32 clinical-type dairy mastitis samples were subjected to traditional bacterial culture,biochemical tests,16S rDNA nucleotide sequence detection and drug sensitivity tests.The results showed that a total of 23 strains of E.coli,12 strains of Streptococcus udder and 5 strains of Klebsiella pneumoniae were isolated and identified without duplication,and the detection rates of the samples were 71.9%(23/32),37.5%(12/32)and 15.6%(5/32),respectively.The drug sensitivity tests showed that most of the E.coli and Streptococcus udder were resistant to penicillin,amoxicillin,ceftazidime,gentamicin and lincomycin serious,resistance rate of 50%~86.9%,to imipenem,tetracycline and erythromycin more sensitive,sensitivity rate of 75%~82.6%.The Klebsiella pneumoniae to most antibiotics bacteria resistance degree is low,to imipenem,amikacin,tetracycline,erythromycin sensitivity rate of 100%.It is suggested that the farm should grasp environmental hygiene,improve biosecurity,and establish a scientific and reasonable treatment plan.

关 键 词：奶牛乳房炎 细菌分离鉴定 药敏试验 

分 类 号：S858.23[农业科学—临床兽医学]