工业大麻miR156基因家族在NaHCO_(3)胁迫下的表达模式及生物信息学分析  

Expression Pattern and Bioinformatics Analysis of miR156 Gene Family of Industrial Hemp Under NaHCO_(3) Stress

作　　者：曹焜 孙宇峰[1] 张晓艳 赵越[1] 边境 朱浩[1] 王盼[1] 韩承伟[1] 孙凯旋[1] 王晓楠[1] CAO Kun;SUN Yufeng;ZHANG Xiaoyan;ZHAO Yue;BIAN Jing;ZHU Hao;WANG Pan;HAN Chengwei;SUN Kaixuan;WANG Xiaonan(Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,Heilongjiang,China)

机构地区：[1]黑龙江省科学院大庆分院,黑龙江大庆163319

出　　处：《中国麻业科学》2024年第1期10-15,46,共7页Plant Fiber Sciences in China

基　　金：黑龙江省自然科学基金项目(LH2022C077);黑龙江省省属科研院所科研业务费项目(CZKYF2022-1-C045);黑龙江省科学院青年创新基金项目(CXJQ2023DQ01)。

摘　　要：工业大麻miR156基因家族在碱性盐胁迫响应过程中发挥重要的调控作用,为探究工业大麻miR156基因家族响应NaHCO_(3)胁迫的分子机制,以盐碱耐受型工业大麻品种火麻一号为供试材料,利用生物信息学和qRT-PCR方法,对miR156基因家族进行分析。结果表明,工业大麻miR156家族基因与芦笋、野草莓、苹果和大豆的亲缘关系较近。2条工业大麻pre-miR156序列,产生一条相同的miR156成熟体,且成熟体的保守性较高。在NaHCO_(3)胁迫后的24 h内,工业大麻miR156基因表达量随胁迫时间的延长呈先升高后降低再升高的趋势。该结果为进一步研究工业大麻miR156基因家族及其靶基因的调控网络提供理论依据。The miR156 gene family of industrial hemp plays an important role in the regulation of response to alkaline salt stress.In order to explore the molecular mechanism of miR156 gene family of industrial hemp in response to NaHCO_(3) stress,this gene family of Huoma No.1(aline-alkali tolerant variety)was analyzed by bioinformatics and qRT-PCR.The results showed that the miR156 family gene of industrial hemp is closely related to asparagus,wild strawberry,apple and soybean.The mature miR156,derived from the two pre-miR156 sequences,exhibited higher conservation.During the 24 hours of NaHCO_(3) stress,the expression of the miR156 gene in industrial hemp initially increased,followed by a decrease and subsequent increase as the stress duration prolonged.The findings of this study established a theoretical foundation for further exploration into the regulatory network of the miR156 gene family and its target genes in industrial hemp under saline-alkali stress.

关 键 词：工业大麻 miR156基因家族 NaHCO_(3)胁迫 

分 类 号：S563.3[农业科学—作物学]