HPLC-UV法测定细辛不同部位中马兜铃酸Ⅰ的含量  

Determination of Aristolochic AcidⅠin Different Parts of Asarum by HPLC-UV

作　　者：张振都[1] 邵郅伟 郝丽珊 王莹[1] 李君梅 张奇 宋志峰[1] ZHANG Zhendu;SHAO Zhiwei;HAO Lishan;WANG Ying;LI Junmei;ZHANG Qi;SONG Zhifeng(Institute of Quality Standard and Testing Technology for Agri-products,Jilin Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment and Agricultural Product Safety of Jilin Province,Changchun 130033,China)

机构地区：[1]吉林省农业科学院农业质量标准与检测技术研究所/吉林省农业环境与农产品安全重点实验室,长春130033

出　　处：《东北农业科学》2024年第1期105-107,共3页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业环境与农产品安全重点实验室建设项目(YDZJ202102CXJD031)。

摘　　要：采用70%甲醇溶液超声提取、HPLC-UV法测定细辛中的马兜铃酸Ⅰ的含量,检出限为0.20 ng,回收率为98.5%~103.5%。检测方法简单,分离度好,灵敏度高,稳定性好。细辛根、茎中未检出马兜铃酸Ⅰ,叶中含量为0.0313~0.0873mg/kg,远低于药典限量要求0.001%,风险很低。The content of aristolochic acid I in asarum was determined by ultrasonic extraction with 70%methanol solution and HPLC-UV method.The detection limit was 0.20 ng.The recovery rate was 98.5%-103.5%.The detection method is simple,with good separation,high sensitivity,and good stability.Aristolochic acid I was not detected in the roots and stems of asarum,and the content in the leaves was 0.0313-0.0873 mg/kg,which was far below the pharmacopoeia limit of 0.001%,and the risk was very low.

关 键 词：HPLC-UV法 细辛 马兜铃酸Ⅰ 

分 类 号：O657.72[理学—分析化学]