麻杏石甘汤对发热模型大鼠的解热作用及H_(2)S/CBS体系的影响  

Antipyretic effect of Maxing Shigan decoction in a rat model of pyrexia and its effect on the hydrogen sulfide/cystathionine-β-synthase system

作　　者：龙媛 贺思雨 朱沁泉 廖艳红 张涤[2] LONG Yuan;HE Siyu;ZHU Qinquan;LIAO Yanhong;ZHANG Di(The First Clinical Traditional Chinese Medicine College of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China)

机构地区：[1]湖南中医药大学第一中医临床学院,湖南长沙410007 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医杂志》2024年第2期163-167,共5页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省政府特批专项“张涤中医儿科临床研究所专项”(99-16);湖南省中医药科研计划项目(B2023020);湖南省教育厅科研项目(22B0359)。

摘　　要：目的:证实麻杏石甘汤对脂多糖(LPS)诱导的发热模型大鼠的解热作用,探究其解热机制及其对大鼠脑组织硫化氢(H_(2)S)/胱硫醚-β-合成酶(CBS)体系的影响。方法:将50只无特定病原体(SPF)SD大鼠随机分为空白组、对照组(蒸馏水)、阿司匹林组(0.125 g/kg阿司匹林肠溶片)、麻杏石甘汤低剂量组(3.125 g/kg麻杏石甘汤)、麻杏石甘汤高剂量组(6.250 g/kg麻杏石甘汤),每组各10只。连续灌胃5 d,末次灌胃1 h后,各给药组予以腹腔注射20μg/kg LPS溶液诱导大鼠出现发热模型,空白组予以等剂量0.9%氯化钠注射液。记录造模后5 h内大鼠肛温的变化情况。末次体温测量后,腹腔注射3%戊巴比妥钠麻醉大鼠,经腹主动脉收集血液,通过酶联免疫法(ELISA)检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、细胞干扰素-α(IFN-α)的水平;取大鼠脑组织,运用实时荧光定量聚合酶链式反应(RT-qPCR)测定脑组织中胱硫醚-β-合成酶(CBS)、硫化氢(H_(2)S) mRNA的表达。结果:与对照组比较,麻杏石甘汤低、高剂量组具有良好的解热效果,且麻杏石甘汤低、高剂量组均能抑制发热大鼠血清中IL-1β、TNF-α的表达,麻杏石甘汤高剂量组能抑制发热大鼠血清IFN-α的表达,差异均有统计学意义(P<0.05)。麻杏石甘汤低、高剂量组CBS、H_(2)S mRNA的含量较对照组显著降低,差异有统计学意义(P<0.01)。结论:麻杏石甘汤具有良好的解热作用,其机制可能与减少血清中炎症因子的释放有关;同时麻杏石甘汤能降低发热模型大鼠脑组织中CBS、H_(2)S mRNA的表达,并可能发挥了其对脑组织的保护作用。Objective:To investigate the antipyretic effect of Maxing Shigan decoction in a rat model of pyrexia induced by lipopolysaccharide(LPS) and related mechanisms,as well as its effect on the hydrogen sulfide(H_2S)/cystathionine-β-synthase(CBS) system in rat brain tissue.Methods:A total of 50 specific pathogen-free Sprague-Dawley rats were randomly divided into blank group,control group(distilled water),aspirin group(0.125 g/kg enteric-coated aspirin tablets),low-dose Maxing Shigan decoction group(3.125 g/kg Maxing Shigan decction),and high-dose Maxing Shigan decoction group(6.250 g/kg Maxing Shigan decoction),with 10 rats in each group.The drugs were given by gavage for 5 days,and at 1 hour after the last administration by gavage,the rats in each administration group were given intraperitoneal injection of 20 μg/kg LPS solution to induce pyrexia in rats,while those in the blank group were given an equal dose of 0.9% sodium chloride injection.The change in rectal temperature was recorded within 5 hours after modeling.After measurement of body temperature for the last time,3% pentobarbital sodium was intraperitoneally injected for anesthesia of rats,and blood was collected via the abdominal aorta.ELISA was used to measure the serum levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and interferon-α(IFN-α).Rat brain tissue was collected,and RT-qPCR was used to measure the mRNA expression levels of CBS and H_(2S) in brain tissue.Results:Compared with the control group,the low-and high-dose Maxing Shigan decoction groups showed a good antipyretic effect.Both low-and high-dose Maxing Shigan decoction could inhibit the expression of IL-1β and TNF-α in serum,and high-dose Maxing Shigan decoction could significantly inhibit the expression of IFN-α in serum(P<0.05).Compared with the control group,the low-and high-dose Maxing Shigan decoction groups showed significant reductions in the mRNA expression levels of CBS and H_2S(P<0.01).Conclusion:Maxing Shigan decoction has a good antipyretic effect,possibly

关 键 词：麻杏石甘汤 发热 炎性因子 硫化氢 胱硫醚-Β-合成酶 

分 类 号：R285.5[医药卫生—中药学]