细胞膜色谱与线粒体膜色谱技术的发展及应用  

Development and Application of Cell Membrane Chromatography and Mitochondrial Membrane Chromatography Technology

作　　者：赵壮 范强 苏武 陈乐融 李华[1] 葛帅 孔宇 龙建纲[1] ZHAO Zhuang;FAN Qiang;SU Wu;CHEN Lerong;LI Hua;GE Shuai;KONG Yu;LONG Jiangang(School of Life Science and Technology,Center for Mitochondrial Biology and Medicine,The Key Laboratory of Biomedical Information Engineering of Ministry of Education,Xi′an 710049,China;School of Pharmacy,Xi′an Jiaotong University,Xi′an 710049,China)

机构地区：[1]西安交通大学,生命科学与技术学院,线粒体生物医学研究所,生物医学信息工程教育部重点实验室,西安710049 [2]西安交通大学,药学院,西安710049

出　　处：《中国药学杂志》2024年第3期210-219,共10页Chinese Pharmaceutical Journal

基　　金：载人航天工程航天医学实验领域项目资助(HYZHXM01023)。

摘　　要：细胞膜色谱法是近年来迅速发展的一种生物亲和色谱方法。其以包覆含受体膜的载体为固定相,将配体与膜受体间相互作用转化为色谱过程。该方法具有生物仿生、高效、低成本等优势,已被广泛用于药物分子筛选和受体与配体相互作用研究。线粒体作为重要的细胞内膜系统,膜结构中分布有多种药物靶点,线粒体膜色谱技术近期也有重要研究进展。笔者综述了细胞膜色谱技术及其应用方面的进展,也对基于细胞膜色谱理论的线粒体膜色谱技术进行简介和展望。Cell membrane chromatography is a bioaffinity chromatography method which has been developing rapidly in recent years,where the carriers coated with receptor-containing biomembrane are packed to stationary phase and the bioaffinity between compound and membrane receptor presents in chromatography process.This method has the advantages of biomimetic,high efficiency and low cost,and has been widely used in drug screening and identifying the affinity between membrane receptor and candidate ligand.Mitochondria,a crucial functional intracellular membrane system,with various drug targets residing in membrane.Recently,significant research advancements have been made in mitochondrial membrane chromatography technology.In this paper,the recent progresses and applications of cell membrane chromatography and mitochondria membrane chromatography are reviewed.

关 键 词：细胞膜色谱 线粒体膜色谱 药物筛选 药物受体相互作用 

分 类 号：R917[医药卫生—药物分析学] Q731[医药卫生—药学]