连翘提取物对肺炎链球菌感染慢性阻塞性肺疾病急性加重期大鼠的干预机制  

Intervention effect and mechanisms of forsythia extracts on rats with Streptococcus pneumoniae infection-induced acute exacerbation of chronic obstructive pulmonary disease

作　　者：王蓓[1] 李皖豫 张珂[2] 林智峰[3] 刘冬[1] WANG Bei;LI Wan-yu;ZHANG Ke;LIN Zhi-feng;LIU Dong(The First Affiliated Hospital of Shihezi University,Shihezi,Xinjiang832008,China;不详)

机构地区：[1]石河子大学第一附属医院呼吸一科,新疆石河子832008 [2]石河子大学药学院,新疆石河子832008 [3]石河子大学第一附属医院肾病内科,新疆石河子832008

出　　处：《中华医院感染学杂志》2024年第5期678-683,共6页Chinese Journal of Nosocomiology

基　　金：新疆建设兵团指导性科技计划基金资助项目(2022ZD008);石河子大学科研计划青年创新培育人才基金资助项目(CXPY202220)。

摘　　要：目的 探究连翘提取物对肺炎链球菌感染慢性阻塞性肺疾病急性加重期(AECOPD)大鼠的干预机制。方法 选取SPF级40只SD大鼠随机分成正常组、模型组、低剂量连翘提取物组、高剂量连翘提取物组,各10只。肺炎链球菌构建肺炎链球菌感染AECOPD大鼠模型,对照组不做处理。建模完成后干预,检测管壁面积与血管总面积比(WA%)、肌性动脉的管壁厚度与血管外径比(WT%);分析肺功能指标、炎性因子水平、肺组织中黏蛋白5AC(MUC5AC)、表皮生长因子受体(EGFR)、磷酸化-丝裂原活化蛋白激酶p38抗体(p38MAPK)的相对表达量。结果 与对照组相比,模型组、连翘提取物低、高剂量组的WT%、WA%、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-8、p38MAPK、MUC5AC、EGFR表达量升高,最大呼吸峰流速(PEF)、用力肺活量(FVC)、第1秒用力呼气容积(FEV1)水平降低(P<0.05);相较于模型组,低、高剂量连翘提取物组的PEF、FEV1、FVC表达升高,WA%、WT%、IL-6、IL-8、TNF-α、MUC5AC、p38MAPK、EGFR表达降低(P<0.05);与连翘提取物低剂量组相比,连翘提取物高剂量组的PEF、FVC、FEV1表达上升,WA%、WT%、TNF-α、IL-8、IL-6、EGFR、MUC5AC、p38MAPK表达下降(P<0.05)。结论 连翘提取物可有效改善肺炎链球菌感染AECOPD大鼠的肺功能,抑制炎症因子表达,调节肺血管重塑,改善预后。OBJECTIVE To explore the intervention mechanisms of forsythia extracts on rats with Streptococcus pneumoniae infection-induced acute exacerbation of chronic obstructive pulmonary disease(AECOPD).METHODS Totally 40 SD rats of SPF grade were randomly divided into the normal group,the model group,the low-dose forsythia extract group and the high-dose forsythia extract group,with 10 rats in each group.S.pneu-moniae was chosen to establish the S.pneumoniae infection-induced AECOPD rat models,the control group was not given any treatment.The intervention was carried out after the modeling was done,the ratio of tube wall area to total vessel area(WA%)and the ratio of muscular artery wall thickness to outer diameter of vessel(WT%)were measured.The levels of pulmonary function indexes and inflammatory factors as well as the relative expres-sion levels of mucoprotein 5AC(MUC5AC),epidermal growth factor receptor(EGFR)and phosphorylation-mito-gen-activated protein kinase p38 antibody(p38MAPK)in lung tissues were observed.RESULTS The WT%,WA%,tumor necrosis factor-α(TNF-α)level,interleukin(IL)-6 level,IL-8 level,and the expression levels of p38MAPK,MUC5AC and EGFR were higher in the model group,the low-dose forsythia extract group and the high-dose forsythia extract group than in the control group;the peak expiratory flow(PEF),forced vital capacity(FVC)and forced expiratory volume in one second(FEV1)were lower in the model group,the low-dose forsythia extract group and the high-dose forsythia extract group than in the control group(P<0.05).The expression levels of PEF,FEV1 and FVC of the low-dose forsythia extract group and the high-dose forsythia extract group were higher than those of the model group;while the WA%,WT%,IL-6 level,IL-8 level,TNF-αlevel,MUC5AC level,p38MAPK level and EGFR level of the low-dose forsythia extract group and the high-dose forsythia extract group were lower than those of the model group(P<0.05);the expression levels of PEF,FVC and FEV1 of the high-dose forsythia extract group were higher than tho

关 键 词：连翘提取物 肺炎链球菌感染 慢性阻塞性肺疾病 急性加重期 肿瘤坏死因子-α 用力肺活量 大鼠模型 

分 类 号：R378.12[医药卫生—病原生物学]