蒙药京大戟奶制前后肠道毒性变化及成分分析  

Changes in Intestinal Toxicity and Component Analysis of Mongolian Medicine Euphorbiae Pekinensis Radix before and after Processing with Milk

作　　者：刘璐 郁红礼[1,2,3] 吴皓[1,2,3] 王欣之[1] 崔小兵[1] 曹玉 林伟豪 沈昌丽 曹杰 LIU Lu;YU Hongli;WU Hao;WANG Xinzhi;CUI Xiaobing;CAO Yu;LIN Weihao;SHEN Changli;CAO Jie(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Key Laboratory of Chinese Medicine Processing,Nanjing 210023,China;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing 210023,China;Nanjing Institute for Food and Drug Control,Nanjing 211198,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药炮制重点实验室,江苏南京210023 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [4]南京市食品药品监督检验院,江苏南京211198

出　　处：《南京中医药大学学报》2024年第3期278-288,共11页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金重点项目(82130116);国家自然科学基金面上项目(82374143)。

摘　　要：目的探究蒙药京大戟奶制前后肠道毒性变化与成分组成变化的相关性。方法小鼠灌胃给予生品京大戟、牛奶浸泡京大戟(奶制)及水浸泡京大戟(水制)的95%乙醇提取物,以粪便含水量、小鼠各肠段炎症因子TNF-α和IL-1β水平为指标,考察京大戟奶制前后的泻下作用及肠道致炎毒性变化;采用LC-MS/MS分析京大戟奶制前后95%醇提取物的成分组成变化。结果与空白组相比,京大戟生品、水制品可导致小鼠粪便含水量及各肠段TNF-α和IL-1β水平显著升高(P<0.05);与生品组相比,奶制品组各指标均显著下降(P<0.05),水制品组则无显著差异,表明水制不能起到减毒作用,辅料牛奶是降低京大戟毒性的关键辅料。质谱分析结果显示,京大戟中共鉴定出50个成分,其中萜类成分38个,酚酸类成分6个,其它成分6个,奶制后各成分含量均有不同程度的下降。对生、制品京大戟质谱数据进行主成分分析(Principal component analysis,PCA)及正交偏最小二乘法-判别分析(Partial least squares discriminant analysis,OPLS-DA)发现,生、制品成分可明显聚为2类,通过t检验筛选出生、制品的13个差异性成分,其中11个是萜类成分,表明京大戟奶制后萜类成分组成发生显著变化。炮制后牛奶辅料残液中检测出来源于京大戟的17个成分,其中16个为萜类成分,表明京大戟萜类成分在炮制浸泡过程中转移至辅料牛奶中。结论京大戟奶制后毒性降低、泻下作用缓和,其减毒机制可能与牛奶浸泡炮制过程中,萜类成分转移至牛奶中,饮片中毒性成分含量降低有关。OBJECTIVE To explore the correlation between changes in intestinal toxicity and changes in component composition of the Mongolian medicine Euphorbiae pekinensis Radix(EPR)before and after processing with milk.METHODS Mice were given 95%ethanol extract of raw EPR,milk-processed EPR and water-processed EPR by gavage.The purgative effect and intestinal inflammatory toxicity changes of EPR before and after milk processing were investigated using the fecal water content and the levels of inflammatory factors TNF-αand IL-1βin each intestinal segment of mice as indicators;LC-MS/MS was used to analyze the composition changes of the 95%alcohol extract of EPR before and after milk processing.RESULTS Compared with the blank group,the raw and water processed products of EPR could significantly increase the water content of mouse feces and the levels of TNF-αand IL-1βin each intestinal segment(P<0.05);compared with the raw product group,all indicators in the milk processing group were significantly reduced(P<0.05),while there was no significant difference in the water processing group,indicating that water processing cannot attenuate toxicity,and the auxiliary material milk is the key auxiliary material to reduce the toxicity of EPR.Mass spectrometry analysis results showed that a total of 50 components were identified in EPR,including 38 terpenoid components,6 phenolic acid components,and 6 other components.The content of each component decreased to varying degrees after milk processing.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed on the mass spectrum data of raw materials and products,and it was found that the components of raw materials and products can be obviously clustered into 2 categories.13 differential components of raw materials and products were screened through t test,and 11 of which were terpene components,indicating that the composition of terpene components changed significantly after milk processing.17 components derived from EPR were d

关 键 词：京大戟 奶制 肠道毒性 萜类成分 差异性成分 

分 类 号：R284[医药卫生—中药学]