我国沿海多鳞四指马[鱼友]群体遗传多样性的fAFLP分析  

fAFLP analysis on population genetic diversity of Eleutheronema rhadinum along China coast

作　　者：赵优[1,2,3] 张涛 赵峰[1] 庄平[1,2] ZHAO You;ZHANG Tao;ZHAO Feng;ZHUANG Ping(Key Laboratory of Fisheries Ecology of the Yangtze Estuary,China Academy of Fishery Sciences,Shanghai200090,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai201306,China;Research Center of General Administration of Customs,Beijing100010,China)

机构地区：[1]农业农村部东海与远洋渔业资源开发利用重点实验室,中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学水产与生命学院,上海201306 [3]海关总署研究中心,北京100010

出　　处：《海洋渔业》2024年第1期53-62,共10页Marine Fisheries

基　　金：公益性行业(农业)科研专项(201203065)。

摘　　要：为了解多鳞四指马[鱼友](Eleutheronema rhadinum)的遗传多样性信息,采用荧光标记扩增片段长度多态性技术(fluorescent amplified fragment length polymorphism,fAFLP)对多鳞四指马[鱼友]8个地理群体214个样本进行扩增和遗传结构分析。结果显示:用9对引物组合共扩增位点493条,其中多态性位点为443条,多态性比例为89.86%;8个地理群体观测等位基因数(Na)、有效等位基因数(Ne)、Nei多样性指数(H)、Shannon信息指数(I)平均值分别为1.6790、1.4672、0.2508和0.3631;群体遗传多样性高低依次为舟山>崇明>启东>东山>广州>湛江>琼海>高雄。应用AMOVA对8个群体的遗传变异来源进行分析得出,总的遗传分化指数F_(st)为0.2372,表明23.72%遗传变异来自于群体间。8个地理群体遗传距离(D)在0.0216~0.1944之间;相似系数(H)在0.8234~0.9786之间,UPGMA聚类得出多鳞四指马[鱼友]群体可划分为4个分支。研究结果可为合理评估和保护多鳞四指马[鱼友]资源、开展遗传育种及开发人工养殖技术等提供基础资料。Eleutheronema rhadinum is a warm marine fish,mainly distributed in the east and south of China coast.E.rhadinum is a valuable marine species with wide adaptability to salinity,fast growth,delicate and delicious meat,and its meat is rich in inorganic salt minerals such as calcium,phosphorus,iron,iodine and vitamins.At present,reports about E.rhadinum are concentrated in the aspects of taxonomy,resource investigation,physiology and morphology.Some research has been done on genetic diversity of E.rhadinum populations,but the sampling area is relatively scattered and not representative.In this paper,samples of 214 individuals from eight geographical populations of E.rhadinum were collected and amplified to analyze the genetic construction by fluorescent amplified fragment length polymorphism(fAFLP)labeling technology.With nine primer combinations,a total of 493 bands were generated,including 443 polymorphic,and the percentage of polymorphic bands was 89.86%.The mean values of the observed number of alleles(Na),effective number of alleles(Ne),Nei genetic diversity index(H),and Shannon information index(I)of the eight populations were 1.6790,1.4672,0.2508 and 0.3631.The order of geographical population genetic diversity was Zhoushan>Chongming>Qidong>Dongshan>Guangzhou>Zhanjiang>Qionghai>Gaoxiong.The AMOVA was used to analyze the genetic variation sources of eight populations,and the total genetic differentiation index(F_(st))was 0.2372,indicating that 23.72%of genetic variation came from inter-population.The genetic distance(D)between eight geographical populations ranged from 0.0216 to 0.1944 and the similarity coefficient(H)was between 0.8234 and 0.9786.Based on research data,the eight populations of E.rhadinum could be divided into four branches by the UPGMA tree.The Chongming population,Qidong population and Zhoushan population were clustered into one branch,Dongshan population and Guangzhou population were clustered into one branch,Gaoxiong group was clustered into one branch,Zhanjiang group and Qionghai group wer

关 键 词：多鳞四指马[鱼友] FAFLP 种质资源 遗传多样性 聚类分析 

分 类 号：S917.4[农业科学—水产科学]