绿木霉NY45对木材腐朽菌的抑制作用及机理研究  

The Inhibitory Effect and Mechanism of Trichoderma virens NY45 on Wood Rotting Fungi

作　　者：熊怡心 方旋 李嘉欣 姜珲 马星霞[1] XIONG Yixin;FANG Xuan;LI Jiaxin;JIANG Hui;MA Xingxia(Research Institute of Wood Industry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院木材工业研究所,北京100091

出　　处：《木材科学与技术》2024年第1期51-57,72,共8页Chinese Journal of Wood Science and Technology

基　　金：国家自然科学基金项目“基于木材微生物多样性分析的木材腐朽机理研究”(31971588)。

摘　　要：为探索木材生物防腐,以绿木霉(Trichoderma virens,NY45株系)为对象,研究其对木材白腐菌彩绒栓菌(Trametes versicolor,Tv)及褐腐菌密粘褶菌(Gloeophyllum trabeum,Gt)的抑制效果并探索抑制机理。结果表明:NY45对两种腐朽菌均有明显的抑制作用,经NY45孢子液处理的毛白杨木材,其耐腐等级从IV级(不耐腐)提升到I级(强耐腐);NY45生长速度远快于木材腐朽菌,可以迅速抢占空间和竞争营养,并能寄生、覆盖、消解木材腐朽菌菌丝,其易挥发性物质对Tv和Gt抑制率分别为18.55%和45.69%,难挥发性代谢产物对两种木材腐朽菌的抑制率均超过90%。NY45有潜力作为木材腐朽生物防治菌进一步开发利用。To develop biocontrol of decay in wood,the antagonistic fungi Trichoderma virens,NY45 strain was studied of its inhibitory effect and mechanism on the white rot fungi Trametes versicolor(Tv)and the brown rot fungi Gloeophyllum trabeum(Gt).The results showed that NY45 had a significant inhibitory effect on both types of rotting fungi.The decay resistance grade of Populus tomentosa treated with NY45 spore solution had improved from stage IV(not decay resistant)to stage I(strongly decay resistant)through laboratory tests.NY45 grew much faster than the wood rotting fungi,so that it prioritize getting the growth space and nutrition of Potato Dextrose Agar culture medium.Mechanistic study showed that NY45 mycelium parasitizes,covers,and disintegrates the wood rotting fungi mycelium.Its volatile substances inhibited Tv and Gt by 18.55%and 45.69%,respectively.Furthermore,the inhibition rates of its non-volatile metabolites on both rot fungi exceeded 90%.NY45 has the potential to be further developed and utilized as a biocontrol fungus against wood decay.

关 键 词：木材防腐 绿木霉 生物防治 拮抗菌 抑制机理 

分 类 号：S792.117[农业科学—林木遗传育种] S782.33[农业科学—林学]