聚合酶链反应方法检测阴道毛滴虫准确性的meta分析  

Accuracy of Polymerase Chain Reaction Based Assays for Trichomonas Vaginitis:A Meta-Analysis

作　　者：周欣 黄翠兰[1] ZHOU Xin;HUANG Cuilan(Department of Obstetrics and Gynecology,the People’s Hospital of Yuechi County,Sichuan Guangan 638300,China)

机构地区：[1]岳池县人民医院妇产科,四川广安638300

出　　处：《现代检验医学杂志》2024年第2期168-174,共7页Journal of Modern Laboratory Medicine

摘　　要：目的系统评价聚合酶链反应检测阴道毛滴虫的应用价值。方法检索中国知网数据库(CNKI)、万方数据知识服务平台、维普数据库和PubMed数据库自建库到2023年5月30日以来基于PCR方法检测阴道毛滴虫的研究文献。经文献筛选、数据提取后,使用软件RevMan 5.4.1和网页服务Meta-Disc 2.0进行Meta分析。结果共纳入36篇文献,16454个临床样本。Meta分析结果显示:聚合酶链反应检测阴道毛滴虫的合并敏感度为0.972(0.943,0.987),合并特异度为0.979(0.968,0.986),诊断比值比为1643.398(673.168,4012.008),阳性似然比为46.209(30.549,69.897)和阴性似然比为0.028(0.013,0.059);亚组分析表明不同性别的临床样本不会影响聚合酶链反应的检测准确性,但不同的PCR检测方法的准确性有差异。结论聚合酶链反应检测阴道毛滴虫的准确性较高,具有良好的应用价值。Objective To investigate the accuracy of polymerase chain reaction(PCR)based assays for Trichomonas Vaginitis.Methods The research literature on the detection of TV based on PCR method since the establishment of the database to May 30,2023 was retrieved from China National Knowledge Infrastructure(CNKI),Wanfang Data Knowledge Service Platform,VIP Database and PubMed Database.After literature screening and data extraction,Meta-analysis was performed using software RevMan 5.4.1 and web service Meta-Disc 2.0.Results A total of 36 literatures and 16454 clinical samples were included.The results of meta-analysis showed that the combined sensitivity and specificity of polymerase chain reaction for the detection of TV were 0.972(0.943,0.987)and 0.979(0.968,0.986),respectively,the diagnostic odds ratio was 1643.398(673.168,4012.008),the positive likelihood ratio was 46.209(30.549,69.897),and the negative likelihood ratio was 0.028(0.013,0.059).Subgroup analysis showed that clinical samples of different genders would not affect the accuracy of polymerase chain reaction,but the accuracy of different PCR detection methods was different.Conclusion The accuracy of polymerase chain reaction in the detection of TV was high and it has good application value.

关 键 词：阴道毛滴虫 聚合酶链反应 META分析 

分 类 号：R382.21[医药卫生—医学寄生虫学] Q503[医药卫生—基础医学]