利用ICP-MS和LA-ICP-TOF-MS探究上海青中铅的富集与转运特征  

Enrichment and Transport Characteristics of Lead in Pakchoi(Brassica rapa var.chinensis(Linnaeus)Kitamura.)were Studied by ICP-MS and LA-ICP-TOF-MS

作　　者：曹敬文 徐照[2] 刘月月 周袁媛 季涛涛 李桃生[2,3] 程雄卫 CAO Jing-wen;XU Zhao;LIU Yue-yue;ZHOU Yuan-yuan;JI Tao-tao;LI Tao-sheng;CHENG Xiong-wei(Institutes of Physical Science and Information Technology,Anhui University,Hefei 230601,China;Institute of Nuclear Energy Safety Technology,Hefei Institute of Physical Sciences,Chinese Academy of Sciences,Hefei 230031,China;University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]安徽大学物质科学与信息技术研究院,安徽合肥230601 [2]中国科学院合肥物质科学研究院核能安全技术研究所,安徽合肥230031 [3]中国科学技术大学,安徽合肥230026

出　　处：《化学试剂》2024年第4期80-87,共8页Chemical Reagents

基　　金：安徽省JMRH项目(E1AS0H205N1)。

摘　　要：利用微波消解-电感耦合等离子体质谱(ICP-MS)和激光剥蚀电感耦合等离子体飞行时间质谱(LA-ICP-TOF-MS)进行定量测定和原位成像,对上海青(Brassica rapa var.chinensis(Linnaeus)Kitamura.)中铅(Pb)的富集与转运特征及其机制进行探究。结果表明:上海青中的Pb含量为根>茎>叶,对Pb的吸收效率为根>茎>叶,转运能力为根到茎>茎到叶;富集系数(BCF)和转运系数(TF)受种植土壤Pb含量、pH值和大气中Pb沉降等因素影响;Pb等元素在叶片中的原位分布图表明Pb主要富集在叶脉中,与铝(Al)和铁(Fe)分布特征相似,与锌(Zn)、铜(Cu)、锰(Mn)、磷(P)、硫(S)和镁(Mg)分布特征存在差异。基于Pb的富集与转运特征及其在叶片中与其他元素原位分布特征的异同,探讨上海青对Pb的吸收、富集和转运机制,为评估Pb潜在的健康危害提供数据支撑。In this study,a combination of microwave digestion,inductively coupled plasma mass spectrometry(ICP-MS),and laser ablation inductively coupled plasma time-of-flight mass spectrometry(LA-ICP-TOF-MS)was utilized to quantitatively determine and perform in situ imaging of lead(Pb)in pakchoi(Brassica rapa var.chinensis(Linnaeus)Kitamura.).The objective was to investigate the enrichment,transport characteristics,and mechanisms of Pb in the plant.The results revealed a distinct distribution pattern of Pb content,with higher concentrations observed in the roots,followed by the stems and leaves of the pakchoi plant.The absorption efficiency showed a similar trend,with roots exhibiting the highest absorption capacity,followed by stems and leaves.Furthermore,the transport capacity indicated a predominant movement of Pb from roots to stems,with subsequent transfer from stems to leaves.The bioconcentration factor and transfer factor were found to be influenced by both the Pb content and the pH value of the planting soil,as well as the deposition of Pb from the atmosphere.Importantly,in situ mapping of Pb and other elements in the pakchoi leaves revealed a preferential enrichment of Pb in the leaf veins,similar to aluminum(Al)and iron(Fe),but different from zinc(Zn),copper(Cu),manganese(Mn),phosphorus(P),sulfur(S),and magnesium(Mg).By analyzing the similarities and differences in the accumulation and transport characteristics between Pb and other elements,this study provided a preliminary understanding of the absorption,enrichment,and transport mechanisms of Pb in pakchoi.These insights offer valuable data for assessing the potential health hazards associated with Pb exposure and contribute to a better understanding of Pb contamination in plants.

关 键 词：PB 上海青 转运 富集 ICP-MS LA-ICP-TOF-MS 

分 类 号：Q81[生物学—生物工程]