芽孢杆菌对鸡粪贮存中氨气排放的影响及其耐受pH、盐分和高氨的能力研究  

Influence of Bacillus strains on ammonia emission during chicken manure storage and their tolerance to pH,salt and ammonium.

作　　者：杜中安 郭凡婧 顾沈怡 戴海洋 李思函 申卫收 DU Zhong-an;GUO Fan-jing;GU Shen-yi;DAI Hai-yang;LI Si-han;SHEN Wei-shou(Jiangsu Atmospheric Environment and Equipment Technology Collaborative Innovation Center,Key Laboratory of High Technology Research on Atmospheric Environment Monitoring and Pollution Control in Jiangsu Province,School of Environmental Science and Engineering,Nanjing University of Information Science and Technology,Nanjing 210044,China;Reading Academy,Nanjing University of Information Science and Technology,Nanjing 210044,China)

机构地区：[1]南京信息工程大学环境科学与工程学院江苏省大气环境监测与污染控制高技术研究重点实验室、江苏省大气环境与装备技术协同创新中心,江苏南京210044 [2]南京信息工程大学雷丁学院,江苏南京210044

出　　处：《环境生态学》2024年第3期74-78,98,共6页Environmental Ecology

基　　金：国家自然科学基金项目(42377311、41771291)资助。

摘　　要：畜禽养殖业是重要的氨气排放源,畜禽粪便贮存过程中排放的大量氨气会加剧空气污染。本研究通过鸡粪室内恒温培养,研究了接种分离筛选的芽孢杆菌对鸡粪贮存过程中氨气排放的影响,并对具有氨气减排效应的芽孢杆菌耐受pH、盐分和高氨的特性进行了分析。结果表明,接种芽孢杆菌H1-10和H2-6相对于不接种对照显著减少了氨气累积排放量,分别减少了8.34%和11.85%(p<0.05)。经16S rRNA测序和NCBI比对分析,H1-10和H2-6均为巨大芽孢杆菌(Bacillus megaterium)。菌株H1-10和H2-6均能够在强碱、高盐分和高氨的纯培养条件下生长代谢。本研究将为利用芽孢杆菌减少畜禽粪便贮存过程中氨气排放提供新思路,为研发具有实用性的微生物菌剂提供菌种资源。Livestock husbandry is the main source of ammonia emissions,and the large amount of ammonia has emitted during manure storage which exacerbates air pollution.In this study,the effect of inoculation with Bacillus isolated from soil on the ammonia emission was investigated during the chicken manure storage through a manure incubation at constant temperature.Moreover,their tolerances to pH,salt and ammonium were determined in pure culture.The results showed that the inoculation with Bacillus H1-10 and H2-6 significantly reduced the cumulative ammonia emission from chicken manure,by 8.34%and 11.85%compared with the non-inoculated control,respectively(p<0.05).Both strains H1-10 and H2-6 were able to grow and metabolize under pure culture conditions of strong alkali,high salinity and high ammonia.This study will provide novel idea to decrease ammonia emission during the storage of livestock and poultry manures with Bacillus strains,and provide microbial resources to develop effective microbial agents.

关 键 词：鸡粪 粪便贮存 巨大芽孢杆菌 氨气排放 耐受性 

分 类 号：X705[环境科学与工程—环境工程]