茄子青枯病抗性遗传效应分析  

Genetic effect of eggplant resistance to bacterial wilt

作　　者：郑洁明 李永强 吴元彩 王鹏[1] 阳燕娟[1] 于文进[1] ZHENG Jie-ming;LI Yong-qiang;WU Yuan-cai;WANG Peng;YANG Yan-juan;YU Wen-jin(College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China)

机构地区：[1]广西大学农学院,广西南宁530004

出　　处：《南方农业学报》2023年第12期3502-3513,共12页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31660568);广西科技重大专项(桂科AA22068088-2)。

摘　　要：【目的】解析茄子青枯病抗性遗传规律,为选育抗病杂交组合、优势育种及挖掘相关抗病基因提供理论支持与技术指导。【方法】以4份茄子种质(Y23、NO21、JA02和SG19)为亲本,通过Griffing完全双列杂交方法对各组合青枯病抗性进行配合力分析;分别构建2个杂交组合SG19×Y23和B1×BC03的6世代遗传群体(P_(1)、P_(2)、F_(1)、F_(2)、BC_(1)P_(1)和BC_(1)P_(2)),采用数量性状主基因+多基因世代联合分析法,对各世代青枯病抗性进行遗传模型分析。【结果】茄子青枯病抗性一般配合力远高于特殊配合力,说明抗性以加性效应为主,其次是非加性效应,受到细胞质基因遗传的影响较小;广义遗传力为69.8%,狭义遗传力为63.3%,说明茄子青枯病抗性的遗传不仅受遗传效应的影响,同时也受环境效应影响;抗青枯病的优良杂交组合为Y23×NO21和NO21×Y23;杂交组合SG19×Y23和B1×BC03的抗性均符合MX2-ADI-ADI遗传模型,即由2对加性—显性—上位性效应主基因+加性—显性—上位性效应多基因控制,以加性效应为主,感病表现为不完全显性,BC_(1)P_(2)群体和F_(2)群体以主基因遗传为主,主基因遗传率为77.112%~88.417%,BC1P1群体以多基因遗传为主,多基因遗传率为35.332%~75.050%。通过发病时期作为病情评价标准估算出的最优遗传模型与通过病级分布估算出的最优模型一致。【结论】在青枯病抗病育种过程中应注意加性效应的利用,尽可能选用青枯病抗性强的亲本进行杂交育种;在后续开展基因定位过程中,可利用2对主基因+多基因控制青枯病抗性的理论作为抗性基因定位的依据,避免抗性基因挖掘的盲目性。【Objective】The genetic rule of bacterial wilt resistance in eggplant was analyzed to provide theoretical support and technical guidance for breeding resistant hybrid combinations,dominant breeding and mining related resistance genes.【Method】Using 4 eggplant germplasm as parents(Y23,NO21,JA02 and SG19),the combining ability analysis of bacterial wilt resistance in each combination was conducted by Griffing complete diallel crossing method.At the same time,the six generation genetic populations(P_((1),P_(2),F_(1),F_(2),BC_(1)P_(1)and BC_(1)P_(2))of two cross combinations SG19×Y23 and B1×BC03were constructed respectively.The combined generation analysis method of major gene+multiple gene for quantitative traits was used to analyze the genetic model of bacterial wilt resistance in each generation.【Result】The general combining ability of resistance to eggplant bacterial wilt was much higher than that of special combining ability,indicating that the resistance was mainly additive effect,followed by non-additive effect,and was less influenced by cytoplasmic genetic inheritance.The generalized heritability was 69.8%,and the narrow heritability was 63.3%,these results indicated that the inheritance of bacterial wilt resistance in eggplant was not only affected by genetic effects,but also by environmental effects.The best cross combinations of resistance to bacterial wilt were Y23×NO21 and NO21×Y23.The resistance of the hybrid combination SG19×Y23 and B1×BC03 was in line with the MX2-ADI-ADI genetic model,that was,it was controlled by two pairs of additive-dominant-epistatic effect major genes+additive-dominant-epistatic effect polygenes.The additive effect was dominant,and the disease susceptibility showed incomplete dominance.The BC_(1)P_(2) and F_(2) populations were mainly inherited by major genes,with a heritability rate of 77.112%to 88.417%.The BC_(1)P_(1) population was mainly inherited by polygenes,with a heritability rate of 35.332%to 75.050%.The optimal genetic model estimated by the onset perio

关 键 词：茄子 青枯病 遗传分析 双列杂交 主基因+多基因 

分 类 号：S436.411[农业科学—农业昆虫与害虫防治]