长梗喉毛花中3种化合物抗烟草花叶病毒活性分析及其对4种防御酶活性的影响  

Effects of three compounds from Comastoma pedunlulatum(Royle ex D.Don)Holub on the resistance to tobacco mosaic virus and activities of four defense enzymes in tobacco

作　　者：程林 李卫 刘逸婷 刘真 陈永对[2] 李干鹏 CHENG Lin;LI Wei;LIU Yi-ting;LIU Zhen;CHEN Yong-dui;LI Gan-peng(School of Ethnic Medicine,Yunnan Minzu University/Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission&Ministry of Education,Kunming,Yunnan 650500,China;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resource and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Kunming,Yunnan 650205,China)

机构地区：[1]云南民族大学民族医药学院/民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南农作物基因资源与种质创制重点实验室,云南昆明650205

出　　处：《南方农业学报》2023年第12期3591-3598,共8页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31860498,31860100);云南省“兴滇英才支持计划”产业创新人才项目(XDYC-CYCX-2022-0065)。

摘　　要：【目的】评价从长梗喉毛花[Comastoma pedunlulatum(Royle ex D.Don)Holub]中分离得到的3种化合物的抗烟草花叶病毒(Tobacco mosaic virus,TMV)活性及其对烟草4种防御酶活性的影响,为研发有效防治TMV的植物源农药提供先导化合物和理论依据。【方法】以心叶烟为试验材料,设化合物处理(当药黄素、高杯喉毛花内酯A和异荭草素)、空白对照(CK)和药剂对照(8%宁南霉素)处理,采用半叶枯斑法测定化合物对TMV侵染的预防保护和钝化作用;以普通烟草栽培品种K326为试验材料,设对照[空白对照(CK)和接种对照(CK+TMV)]、宁南霉素[药剂对照(PC)]、当药黄素、高杯喉毛花内酯A和异荭草素处理,利用防御酶检测试剂盒测定各处理烟草叶片中多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。【结果】先施用被试物后接种TMV,当药黄素、高杯喉毛花内酯A和异荭草素在100μg/mL时均减少TMV在心叶烟上的枯斑数目,对TMV的抑制率分别为50.05%、57.55%和61.73%,对TMV侵染均具有一定的预防保护作用,对照药剂宁南霉素对TMV的抑制率为47.75%;TMV与被试物溶液混合接种心叶烟时,当药黄素和高杯喉毛花内酯A对TMV的抑制率分别为51.65%和45.66%,对TMV侵染有较好的钝化作用。分别施用当药黄素、高杯喉毛花内酯A和异荭草素后,烟草叶片防御酶PPO活性分别在处理后24、12和24 h达最大值;PAL活性均在处理后72 h达最大值;POD活性分别在处理后24、48和72 h达最大值。PPO、PAL和POD活性在最大值时与CK均呈显著差异(P<0.05),而SOD活性在最大值时与CK无显著差异(P>0.05)。【结论】分离自长梗喉毛花的3种化合物当药黄素、高杯喉毛花内酯A和异荭草素使用浓度为100μg/mL时对TMV均有较好的预防保护活性,具有作为抗TMV植物源农药或先导化合物的潜力。【Objective】To evaluate the anti-tobacco mosaic virus(TMV)activity of three compounds isolated from Comastoma pedunlulatum(Royle ex D.Don)Holub and their effects on the activity of four tobacco defense enzymes,which could provide lead compounds and theoretical basis for the research and development of effective control of TMV plant pesticides.【Method】Nicotiana glutinosa was used as the materials,there were three groups,which were com‐pounds treatment group(swertisin,comtraide A and isoorientin),negative control(CK)and positive control(8%ning‐nanmycin).The protective and inactivation effects of the compounds on TMV infection were determined by half-leaf method.Using tobacco cultivar K326 as the materials,control group[negative control(CK)and TMV-inoculated control(CK+TMV)],ningnanmycin treatment group[positive control(PC)]and swertisin,comtraide A and isoorientin treat‐ment were set.The activities of polyphenol oxidase(PPO),phenylalanine ammonlyase(PAL),superoxide dismutase(SOD)and peroxidase(POD)in tobacco leaves were determined by defense enzyme detection kit.【Result】Swertisin,comtraide A and isoorientin could reduce the lesion numbers of TMV on N.glutinosa plants when the tobacco leaves were first applied with compounds at 100μg/mL and then inoculated with TMV,showing good protective effects against TMV infection,with the inhibition rates of 50.05%,57.55%and 61.73%,respectively,whereas the inhibition rate of ningnan‐micin against TMV was only 47.75%.When the TMV were mixed with compounds and then inoculated the plant leaves,swertisin and comtraide A also had good inactivation effects against TMV,whose inhibition rates were 51.65%and 45.66%,respectively.After application of swertisin,comtraide A and isoorientin,the maximum activities of defense en‐zymes PPO were reached after 24,12 and 24 h,respectively;the maximum activities of PAL were reached after 72 h;the maximum activities of defense enzymes POD were reached after 24,48 and 72 h,respectively.The activities of PPO,PAL and POD were sign

关 键 词：长梗喉毛花 黄酮类 裂环环烯醚萜苷类 烟草花叶病毒 防御酶活性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]