归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用  

Regulation of compound preparation of Guiqi Yimu on oxidized lipids in blood of postpartum dairy cows

作　　者：毛永霞 王菲菲 孔维怡 岳康 王瑞玲 刘欣 丁辉 郭延生[1] MAO Yong-xia;WANG Fei-fei;KONG Wei-yi;YUE Kang;WANG Rui-ling;LIU Xin;DING Hui;GUO Yan-sheng(College of Animal Science and Technology,Ningxia University,Yinchuan,Ningxia 750021,China)

机构地区：[1]宁夏大学动物科技学院,宁夏银川750021

出　　处：《南方农业学报》2023年第12期3697-3707,共11页Journal of Southern Agriculture

基　　金：宁夏自然科学基金项目(2023AAC03103)。

摘　　要：【目的】探究归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用,以期进一步明确归芪益母复方制剂抗炎和抗氧化的作用机制,为归芪益母复方制剂在产后奶牛保健与疾病防治中的应用提供参考依据。【方法】选取体况相近、2~3胎次的健康待产荷斯坦奶牛15头,将其随机分成对照组(n=7)和试验组(n=8),试验组奶牛产后灌服归芪益母复方制剂500 mL/头,连续灌服6 d,对照组奶牛每日灌服等体积饮用水。产后7 d晨饲前于尾静脉采集血液,制备血浆样本,采用超高效液相色谱—串联质谱(UPLC-MS/MS)技术定量检测2组奶牛血浆中氧化脂质含量,分析2组奶牛血浆氧化脂质代谢轮廓的变化规律,筛选产后奶牛血液中的差异氧化脂质并进行代谢通路富集分析。【结果】2组奶牛血浆中共鉴定出44种氧化脂质,其中花生四烯酸及其代谢物18个、亚麻酸及其衍生物12个、二十碳五烯酸及其衍生物9个、二十二碳六烯酸及其衍生物4个、二十二碳五烯酸及其衍生物1个。产后7 d,试验组奶牛血浆中8种氧化脂质代谢轮廓较对照组发生显著变化(P<0.05,下同),其中5种脂质分子显著上调(VIP≥1,FC≥2),包括12-HETE、11-HETE、15-HETE、5-HETE和15-oxoETE;3种脂质分子显著下调(VIP≥1,FC≤0.5),包括20-HETE、8,9-EET和TXB2。代谢通路富集分析结果表明,差异脂质分子主要富集在花生四烯酸代谢通路、TRP通道的炎症介质调节通路和PPAR信号通路。【结论】归芪益母复方制剂主要通过花生四烯酸代谢、TRP通道的炎症介质调节和PPAR信号通路来调节产后奶牛脂质代谢,从而发挥抗炎和抗氧化作用。【Objective】To investigate the regulation of oxidized lipids in the blood of postpartum dairy cows by Guiqi yimu compound preparation,with a view to further clarifying the anti-inflammatory and antioxidant mechanisms of Guiqi Yimu compound preparation,and to provide reference basis for the application of Guiqi yimu compound preparation in the health care and disease control of postpartum dairy cows.【Method】Fifteen healthy Holstein cows with similar body condition and 2-3 parities were selected and randomly divided into the control group(n=7)and the experimental group(n=8).The cows in the experimental group were dosed with Guiqi Yimu compound formulation 500 mL/cow after parturi‐tion for 6 consecutive d,while the cows in the control group were dosed with an equal volume of drinking water every day.Blood was collected from the tail vein before morning feeding 7 d postpartum and plasma samples were prepared.Ul‐tra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to tandem quantitatively de‐tect the content of oxidized lipids in the plasma of two groups of dairy cows,analyze the changing law of the metabolic profile of oxidized lipids in the plasma of two groups of dairy cows,and screen for the differences of oxidized lipids in the blood of postpartum dairy cows and carry out the analysis of metabolic pathway enrichment. 【Result】A total of 44 oxi‐dized lipids were identified in the plasma of 2 groups of cows, including 18 arachidonic acids and its metabolites, 12 lino‐lenic acids and its derivatives, 9 eicosapentaenoic acids and its derivatives, 4 docosahexaenoic acids and its derivatives, and 1 docosapentaenoic acid and its derivatives. At 7 d postpartum, 8 oxidized lipid metabolic profiles of cows plasma in the experimental group changed significantly compared with the control group(P<0.05, the same below), of which 5 oxi‐dized lipids molecules were significantly up-regulated(VIP≥1,FC≥2), including 12-HETE, 11-HETE, 15-HETE, 5-HETE and 15-oxoETE, and three we

关 键 词：奶牛 归芪益母复方制剂 脂质组学 氧化脂质 代谢通路 

分 类 号：S853.73[农业科学—临床兽医学]