一株罕见G4P[6]-I5-A8基因型人轮状病毒全基因组序列分析  

Whole-genome analysis of a rare human rotavirus strain of genotype G4P[6]-I5-A8

作　　者：车如意 周杰英[2] 范佳欣 唐晓苹 董梦洁 熊光萍 孙晓曼[1] 王宏[1] 章青[1] 庞立丽[1] 孔翔羽[1] 李丹地[1] Che Ruyi;Zhou Jieying;Fan Jiaxin;Tang Xiaoping;Dong Mengjie;Xiong Guangping;Sun Xiaoman;Wang Hong;Zhang Qing;Pang Lili;Kong Xiangyu;Li Dandi(National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Beijing 102206,China;Chenzhou First Municipal People’s Hospital,Chenzhou 423000,China)

机构地区：[1]传染病溯源预警与智能决策全国重点实验室、中国疾病预防控制中心病毒病预防控制所、国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [2]郴州市第一人民医院,423000

出　　处：《国际病毒学杂志》2024年第1期17-21,共5页International Journal of Virology

摘　　要：目的研究一株G4P[6]基因型A组轮状病毒(group A rotavirus,RVA)CZ079的全基因组特征及进化规律.方法利用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)对CZ079的VP7、VP4、VP6、NSP1节段分别进行RT-PCR扩增,通过在线RVA自动分型工具对其分型,采用DNAstar5.1和Mega 11.0软件对各节段进行同源性及遗传进化分析.结果CZ079毒株基因型为G4-P[6]-I5-R 1-C1-M1-A8-N1-T1-E1-H1.VP6为I5型,NSP1为A8型.CZ079与LLR^(TM)、RotaTeq^(TM)和Rotarix^(TM)三种疫苗株在VP7的抗原表位中7-1b区域均存在氨基酸差异;VP4蛋白裂解后的VP8*抗原表位中8-1、8-2、8-3、8-4、5-1区域存在较大差异.结论2022年RVA毒株CZ079为罕见的G4P[6]-I5-A8型,推测该基因型RVA是由越南等东南亚地区传入中国,与中国RVA发生重配产生的新型重配毒株.Objective To study the whole genome characteristics and evolutionary pattern of a genotype G4P[6]strain of group A rotavirus(RVA)CZ079 in China.Methods The genomic segments of CZ079,VP7,VP4,VP6 and NSP1 were amplified by reverse transcription polymerase chain reaction(RT-PCR),and the genotyping used online RVA automatic typing tool.DNAstar 5.1 and MEGA 11.0 were used in homology and genetic evolution analyses.Results The genotype of the CZ079 strain was G4-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1.The typing by VP6 was type I5,and type A8 by NSP1.The amino acid differences were found between CZ079 and all three vaccine strains of LLR™,RotaTeq™and Rotarix™in the region 7-1b within the antigenic epitopes of VP7.After degradation of VP4,the 8-1,8-2,8-3,8-4 and 5-1 regions of VP8*antigenic epitopes showed large differences.Conclusions The RVA strain CZ079 detected in China in 2022 was a rare strain of genotype G4P[6]-I5-A8.It is hypothesized that this genotype of RVA was imported to China from Vietnam and other Southeast Asian regions,and formed a new reassortment strain by reassortment with RVA strains in China.

关 键 词：A组轮状病毒 基因型 重配株 全基因组 抗原表位 

分 类 号：R511[医药卫生—内科学]