芩楼合剂对兔耳痤疮模型病理变化及血清睾酮、TNF-α、IL-6、IL-1β的影响  

Effects of Qinlou Mixture on Pathological Changes and Serum Testosterone,TNF-α,IL-6 and IL-1βof Rabbit Ear Acne Model

作　　者：秦华萍 林燕 赵丽娟 王金容 杨雪松 叶建州 伍迪 QIN Huaping;LIN Yan;ZHAO Lijuan;WANG Jinrong;YANG Xuesong;YE Jianzhou;WU Di(Qujing Maternal and Child Health Hospital,Qujing 655000,China;Yunnan University of Chinese Medicine,Kunming 650500,China;The First Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650021,China)

机构地区：[1]曲靖市妇幼保健院,云南曲靖655000 [2]云南中医药大学,云南昆明650500 [3]云南中医药大学第一附属医院,云南昆明650021

出　　处：《云南中医药大学学报》2024年第1期51-56,共6页Journal of Yunnan University of Chinese Medicine

基　　金：云南省科技厅中医联合专项面上项目(202101AZ070001-079)。

摘　　要：目的观察芩楼合剂对兔耳痤疮模型病理变化及血清中睾酮、TNF-α、IL-6、IL-1β含量水平的影响,探讨其治疗痤疮的作用机制。方法将42只兔子随机分为芩楼合剂高、中、低剂量组,正常组及模型对照组,阳性药物组(异维A酸软胶囊),每组7只,经涂抹煤焦油和接种痤疮丙酸杆菌造模成功后进行干预,灌胃、浓缩给药14 d后,ELISA法检测睾酮、TNF-α、IL-6、IL-1β的含量;以及HE镜下观察模型病理变化。结果实验组给药后与模型组比较,血清睾酮、TNF-α、IL-6、IL-1β含量水平皆有降低,其中芩楼合剂高剂量组、芩楼合剂中剂量组均降低显著(P<0.01);芩楼合剂低剂量组下降的差异无统计学意义(P>0.05),显示了芩楼合剂的药物效应与浓度成正相关。HE切片观察发现芩楼合剂可以减轻兔耳毛囊角栓,减少炎症细胞浸润。结论芩楼合剂治疗痤疮作用机制可能与调控痤疮的相关炎症介质和睾酮密切相关。Objective To observe the pathological changes and serum levels of testosterone,TNF-α,IL-6 and IL-1βof Qinlou Mixture on rabbit ear acne model,and to explore the mechanism of Qinlou Mixture in the treatment of acne.Methods Forty-two rabbits were randomly divided into Qinlou mixture high-dose,medium-dose and low-dose groups,normal group,model control group and drug control group,with 7 rabbits in each group.After being coated with coal tar and inoculated with propionobacter acne for modeling,intervention was carried out.After 14 days of intragastric administration,serum levels of testosterone,TNF-α,IL-6 and IL-1βwere determined by ELISA.The pathological changes of the model were observed under HE microscope.Results Compared with model group,the levels of serum testosterone,TNF-α,IL-6 and IL-1βin experimental group were decreased after administration,and the levels of qinlou Mixture high dose group and Qinlou Mixture medium dose group were significantly decreased(P<0.01).There was no significant difference in the decrease of qinlou Mixture low dose group(P>0.05),indicating that the drug effect of Qinlou Mixture was positively correlated with the concentration.HE section observation showed that Qinlou Mixture can reduce the rabbit ear follicular plug,reduce the infiltration of inflammatory cells.Conclusion The mechanism of Qinlou Mixture in the treatment of acne may be closely related to the inflammatory mediators and testosterone.

关 键 词：芩楼合剂 痤疮模型 病理变化 炎症因子 睾酮 

分 类 号：R275.9[医药卫生—中西医结合]