糖尿病肾病小鼠CD4^(+)T细胞的脂质组学研究  

Study on lipidomics of CD4^(+)T cells in mice with diabetic kidney disease

作　　者：张小雨 谭海波 黄敏仪 贝伟剑 杨祎琦 ZHANG Xiaoyu;TAN Haibo;HUANG Minyi;BEI Weijian;YANG Yiqi(Institute of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学中医药研究院,广州510006

出　　处：《中国免疫学杂志》2024年第3期471-477,共7页Chinese Journal of Immunology

基　　金：广东省医学科学技术研究基金项目(B2020023)。

摘　　要：目的:初步探讨糖尿病肾病(DKD)小鼠CD4^(+)T免疫细胞在脂质组学方面的差异,筛选出具有生物学意义的差异代谢产物。方法:先采用CD4 (L3T4) MicroBeads免疫磁珠法分离BKS.Cg-Dock7^(m+/+)Lepr^(db)/J自发性DKD小鼠脾脏CD4^(+)T免疫细胞;流式细胞术鉴定CD4^(+)T免疫细胞纯度,LC-MS/MS技术检测CD4^(+)T免疫细胞非靶向脂质组学,对差异代谢产物进行分析。结果:LC-MS法检测出463个代谢产物;PCA和OPLS-DA分析显示代谢组分明显分离;筛选出24种差异代谢物。KEGG及富集分析可知差异代谢物涉及甘油磷脂代谢紊乱。结论:CD4^(+)T细胞磷脂类代谢与DKD的发生密切相关,靶向DKD CD4^(+)T细胞的磷脂类代谢可能是DKD治疗的新方向。Objective:To investigate the lipidomics differences of CD4^(+)T immune cells in diabetic kidney disease(DKD)mice,and screen out the differential metabolites with biological significance.Methods:CD4(L3T4)MicroBeads immunomagnetic beads were used to isolate CD4^(+)T immune cells from spleen of BKS.Cg-Dock7^(m+/+)Lepr^(db)/J mice with spontaneous DKD;the purity of CD4^(+)T cells were identified by flow cytometry.The non-targeted lipidomics of CD4^(+)T cells were detected by LC-MS/MS,and the differ-ential metabolites were analyzed.Results:A total of 463 metabolites were detected by LC-MS.PCA and OPLS-DA analysis showed that the metabolic components were significantly separated;twenty-four differential metabolites were screened out.KEGG and enrich-ment analysis showed that the differential metabolites involved in the disorder of glycerol phospholipid metabolism.Conclusion:Phos-pholipid metabolism of CD4^(+)T cells is closely related to the occurrence of DKD.Phospholipid metabolism targeting DKD CD4^(+)T cells in DKD may be a new direction of DKD treatment.

关 键 词：CD4^(+)T细胞 糖尿病肾病 脂质组学 磁珠分选 流式细胞术 

分 类 号：R392.6[医药卫生—免疫学]