对银屑病患者外周血中性粒细胞胞外诱捕网形成的观察  

Observation on formation of neutrophil extracellular traps in peripheral blood of patients with psoriasis

作　　者：屈小燕 陈盼 倪文琼 肖辉 QU Xiaoyan;CHEN Pan;NI Wenqiong;XIAO Hui(Department of Dermatology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou 450007,China)

机构地区：[1]郑州大学附属郑州中心医院皮肤科,郑州450007

出　　处：《中国免疫学杂志》2024年第3期599-603,609,共6页Chinese Journal of Immunology

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20191048)。

摘　　要：目的:探索中性粒细胞胞外诱捕网(NETs)在银屑病患者病情评估中的临床应用价值。方法:收集63例银屑病患者及27例健康体检者外周血各2 ml,密度梯度离心法分离外周血中性粒细胞,免疫荧光染色观察NETs形成情况,计算NETs数量及荧光强度,并与临床分型、疾病严重程度及炎症指标进行相关性分析。收集其中5例银屑病患者皮损组织,免疫荧光法观察NETs。结果:银屑病患者外周血自发NETs的比例及荧光强度显著高于健康对照者,且与银屑病PASI评分呈正相关。合并代谢异常或TNF-α异常的银屑病患者中NETs生成高于不合并代谢异常或TNF-α正常的银屑病患者。银屑病皮损中的NETs表达增多。结论:NETs水平与银屑病、银屑病合并代谢异常患者的疾病严重程度相关,或可反映银屑病及银屑病代谢共病的炎症状态用于疾病评估。Objective:To explore the clinical application value of neutrophil extracellular traps(NETs)in the evaluation of psoriasis patients.Methods:Peripheral blood(2 ml)of 63 patients with psoriasis and 27 healthy controls were collected,neutrophils were isolated by density gradient method,and the formation of NETs was observed by immunofluorescence staining.Calculated the pro-portion and fluorescence intensity of NETs,and analyzed their correlation with clinical classification,severity and serological indica-tors.Skin lesions of 5 patients with psoriasis were collected,and the NETs were observed by immunofluorescence method.Results:Proportion and fluorescence intensity of spontaneous NETs in patients with psoriasis were significantly higher than those in healthy con-trols,and were positively correlated with the PASI scores.Production of NETs in psoriasis patients with metabolic abnormalities or TNF-αabnormalities was higher than that in psoriasis patients without metabolic abnormalities or TNF-αnormalcy.Expressions of NETs in psoriatic lesions were increased.Conclusion:Levels of NETs are correlated with disease severity of psoriasis and patients with metabolic abnormalities,and may be used as a clinical indicator to reflect the inflammatory state of psoriasis and metabolic comor-bidity of psoriasis for disease evaluation.

关 键 词：银屑病 中性粒细胞胞外诱捕网 疾病严重性 代谢相关共病 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]