温经汤对大鼠子宫内膜异位症SPARC介导的上皮-间质转化的影响  

Effect of Wenjing Decoction on SPARC mediated epithelial‑mesenchymal transformation in rats with endometriosis

作　　者：庄梦斐[1] 李盼盼 丁楠 施佑瑾 朱琳 曹阳[1] 谭丽[1] 黄圣惠 杭斐 张婷婷[1] 孙兆贵 ZHUANG Mengfei;LI Panpan;DING Nan;SHI Youjin;ZHU Lin;CAO Yang;TAN Li;HUANG Shenghui;HANG Fei;ZHANG Tingting;SUN Zhaogui(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Traditional Chinese Medicine Hospital of Kunshan,Kunshan,Jiangsu 215300,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Institute for Biomedical and Pharmaceutical Technologies,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]昆山市中医医院,江苏昆山215300 [3]上海中医药大学,上海201203 [4]上海市生物医药技术研究院,上海200032

出　　处：《上海中医药杂志》2024年第4期9-18,共10页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82104906,82074475);上海市卫健委上海市进一步加快中医药传承创新发展三年行动计划项目[ZY(2021-2023)0302];上海市卫健委海派中医流派传承创新团队建设项目(2021LPTD-002);国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023065)。

摘　　要：目的探讨温经汤对子宫内膜异位症(EMT)大鼠模型富含半胱氨酸的酸性分泌蛋白(SPARC)介导的上皮-间质转化的影响。方法采用手术自体移植子宫内膜块法建立EMT大鼠模型,将建模成功的40只大鼠随机分为模型组(给予质量分数为0.9%的氯化钠溶液10 mL/kg)、孕三烯酮组(给予孕三烯酮溶液0.5 mg/kg)以及温经汤低、中、高剂量组(分别给予温经汤免煎颗粒剂8.19 g/kg、16.38 g/kg、32.76 g/kg),每组各8只,另取7只未手术大鼠作为正常对照组(给予质量分数为0.9%的氯化钠溶液10 mL/kg)。所有大鼠均连续灌胃给药3周后进行取材。腹主动脉取血,采用酶联免疫吸附试验(ELISA)法检测血清中SPARC水平。电子游标卡尺测量异位病灶体积,计算生长抑制率。取下异位病灶组织(正常对照组取下双侧子宫组织)采用免疫组织化学法检测内膜组织中SPARC、锌指转录因子(Snail)、E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)的表达,采用Western blot法和RT-qPCR法测定内膜组织中上述因子的蛋白和mRNA相对表达量。结果各治疗组异位病灶的生长抑制率与模型组比较差异均有统计学意义(P<0.05)。与正常对照组比较,模型组血清中SPARC水平显著升高(P<0.05),各治疗组血清中SPARC水平均明显低于模型组(P<0.05)。模型组异位内膜中SPARC、Snail、Vimentin呈高表达,E-cadherin呈低表达,经温经汤治疗后,异位内膜中SPARC、Snail、Vimentin的阳性表达减少,E-cadherin的阳性表达增加。温经汤中、高剂量组和孕三烯酮组异位内膜中SPARC、Snail、Vimentin的蛋白相对表达量均明显低于模型组(P<0.05),E-cadherin的蛋白相对表达量显著高于模型组(P<0.05)。温经汤高剂量组和孕三烯酮组异位内膜中Snail、Vimentin mRNA相对表达量均较模型组显著降低(P<0.05),E-cadherin mRNA相对表达量明显高于模型组(P<0.05)。结论温经汤可以降低EMT大鼠模型血清中SPARC水平,下调异位内膜�Objective To investigate the effects of Wenjing Decoction on secreted protein acidic in rich cysteine(SPARC)mediated epithelial-mesenchymal transformation in rats with endometriosis(EMT).Methods The model was established by surgical autotransplantation of endometrial block.Forty successfully modeled rats were randomly divided into model group(n=8,0.9%NaCl solution 10 mL/kg),low-dose Wenjing Decoction group(n=8,Wenjing Decoction granules 8.19 g/kg),medium-dose Wenjing Decoction group(n=8,Wenjing Decoction granules 16.38 g/kg),high-dose Wenjing Decoction group(n=8,Wenjing Decoction granules 32.76 g/kg)and gestrinone group(n=8,gestrinone solution 0.5 mg/kg),and the non operated rats were taken as normal group(n=7,0.9%NaCl solution 10 mL/kg).All rats were anatomized and the samples were obtained after 3 weeks of continuous intragastric administration.Blood was taken from abdominal aorta and the level of SPARC in serum was detected by ELISA assay.The volume of ectopic lesions was measured with electronic vernier caliper,and the growth inhibition rates were calculated.The expressions of SPARC,zinc finger transcription factor(Snail),E-cadherin and Vimentin in endometrium were detected by immunohistochemistry,and the relative expressions of these factors in endometrium were measured by Western blot and RT-qPCR methods.Results Compared with the model group,the growth inhibitory rates of ectopic lesions in each treatment groups were significantly elevated(P<0.05).The level of SPARC in serum in the model group was obviously increased compared with that in the normal group(P<0.05),and the levels of SPARC in serum in each treatment groups were significantly declined(P<0.05).In the model group,SPARC,Snail and Vimentin were highly expressed in ectopic endometrium,while E-cadherin was low expressed.After treatment with Wenjing Decoction,the positive expressions of SPARC,Snail and Vimentin in ectopic endometrium decreased,and the positive expression of E⁃cadherin increased.The relative expressions of SPARC,Snail,Vimentin protei

关 键 词：子宫内膜异位症 温经汤 上皮-间质转化 大鼠模型 经典名方 中药研究 

分 类 号：R285.5[医药卫生—中药学]