基于UPLC-Q-Exactive Orbitrap-MS的知母-黄柏药对入血成分分析  

Analysis of Absorbed Components of Rhizoma Anemarrhenae-Cortex Phellodendri Chinesis Herb-Pair Based on UPLC-Q-Exactive Orbitrap-MS

作　　者：夏天利 王岩 王迪[1] 赵德萍 苏婷 赵继会 雷霞 张宁[1,3] XIA Tianli;WANG Yan;WANG Di;ZHAO Deping;SU Ting;ZHAO Jihui;LEI Xia;ZHANG Ning(Jiamusi University,Jiamusi 154007,China;School of Pharmaceutical Sciences,Hunan University of Medicine,Huaihua 418000,China;School of Pharmacy,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Institute of Traditional Chinese Medicine,Wuxi Traditional Chinese Medicine Hospital,Wuxi 214071,China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]湖南医药学院药学院,湖南怀化418000 [3]黑龙江中医药大学药学院,哈尔滨150040 [4]无锡市中医医院中医药研究所,江苏无锡214071

出　　处：《中国药学杂志》2024年第4期330-337,共8页Chinese Pharmaceutical Journal

基　　金：黑龙江省自然科学基金项目资助(LH2020H097);黑龙江省中医药科研项目资助(ZHY202088);江苏省中医退行性骨关节病临床医学创新中心资助项目资助(苏中科教[2021]4号)。

摘　　要：目的以正常家兔为实验对象,探讨知母-黄柏药对口服吸收后的入血成分。方法采用相当于生药量4.5 g·kg^(-1)的知母-黄柏水煎液灌胃家兔,每日1次,连续7 d,末次给药后取家兔含药血清,利用超高效液相色谱-四级杆-静电场轨道阱高分辨质谱联用技术(UPLC-Q-Exactive Orbitrap-MS),在正、负两种离子模式下,根据保留时间、精确相对分子质量、二级质谱裂解碎片,确定入血成分。结果家兔含药血清中共鉴别出54个入血成分,包括46个原型入血成分,包括生物碱类、黄酮类、皂苷类、糖苷类、酚酸类、苯丙素类、内酯类、酚类衍生物;9个代谢产物,分别来源于小檗碱、药根碱、小檗红碱、芒果苷。结论知母-黄柏药对中含有46个可以入血的化学成分,还可能含有4个化学成分在体内代谢为9个代谢产物。OBJECTIVE To study the components of Rhizoma Anemarrhenae-Cortex Phellodendri Chinesis(RA-PC)herb-pair absorbed into blood in normal rabbits.METHODS Rabbits were fed with the decoction of RA-PC herb pair(4.5 g·kg^(-1))once a day for 7 days,then blood samples were taken.High-performance liquid chromatography-quadrupole-electrostatic field orbital trap(UPLC-Q-Exactive Orbitrap-MS)technique was applied for the analysis,and fragments were split in both positive and negative ion modes.The compositions into blood were determined according to retention time,accurate relative molecular mass,and secondary mass spectra.RESULTS A total of 54 components were identified in the drug-containing serum of rabbits,including 46 prototype components,which were alkaloids,flavonoids,saponins,glycosides,phenolic acids,phenylpropanoids,lactones,and phenolic derivatives.The nine metabolites were derived from berberine,jatrorrhizine,berberrubine,and mangiferin,respectively.CONCLUSION RA-PC contains 46 chemical components that can enter blood of normal rabbits,and may also contains four chemical components which are metabolized into nine metabolites.

关 键 词：知母 黄柏 药对 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术 入血成分 

分 类 号：R917[医药卫生—药物分析学]