升降理肺消瘤汤对Lewis肺癌小鼠免疫炎性反应和JAK2/STAT3信号通路的影响  

Effect of Shengjiang Lifei Xiaoliu Decoction(升降理肺消瘤汤)on Immune Inflammatory Response and JAK2/STAT3 Signal Pathway in Lewis Lung Cancer Mice

作　　者：梁帅 尹怡[2] 刘湘花[1] 汪保英[1] 骆文龙 龙云凯 任振杰 王祥麒[2] LIANG Shuai;YIN Yi;LIU Xianghua;WANG Baoying;LUO Wenlong;LONG Yunkai;REN Zhenjie;WANG Xiangqi(Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;The Third Affiliated Hospital to Henan University of Chinese Medicine,Zhengzhou 450008,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第三附属医院,河南郑州450008

出　　处：《辽宁中医药大学学报》2024年第4期27-32,共6页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：河南省中医药科学研究专项(2022ZYZD13)。

摘　　要：目的探讨升降理肺消瘤汤对Lewis肺癌小鼠免疫和炎性反应的调控作用,以及对JAK2/STAT3信号通路的影响。方法适应性喂养C57BL/6J雄性小鼠1周,其中空白组8只,荷瘤组60只。荷瘤组小鼠注射Lewis肺癌细胞造模成功后,将瘤体肉眼可见的荷瘤小鼠分为模型组、PD-1抑制剂组、升降理肺消瘤汤(XLT)低剂量组、XLT中剂量组、XLT高剂量组、XLT中剂量联合PD-1抑制剂组(联合用药组),每组8只。空白组和模型组每日0.4 mL/20 g生理盐水灌胃;PD-1抑制剂组每3 d腹腔注射100μg信迪利单抗;XLT低、中、高剂量组每日0.4 mL/20 g相应浓度中药灌胃;联合用药组每日0.4 mL/20 g中浓度中药灌胃,每3 d腹腔注射100μg信迪利单抗,各组连续给药14 d。最后一次给药的24 h后,称量记录小鼠体质量,摘眼球取血,颈椎脱臼法处死小鼠,比较各组小鼠抑瘤率、去瘤体质量、胸腺指数和脾指数;ELISA法检测血清中白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、IL-6浓度水平;Western Blot检测Janus激酶2(JAK2)、p-JAK2、信号转导和转录激活因子3(STAT3)、p-STAT3蛋白表达水平。结果与模型组比较,各用药组小鼠平均瘤重均较少,PD-1抑制剂组、XLT中、高剂量组瘤重明显减少(P<0.01);PD-1抑制剂组和XLT中剂量组去瘤体质量增加(P<0.01);各用药组小鼠胸腺指数和脾指数均显著增加(P<0.01);PD-1抑制剂组、XLT中剂量组及联合用药组IL-2水平显著升高(P<0.05),各用药组小鼠IFN-γ水平均明显升高(P<0.05);除XLT低剂量组,各用药组TNF-α和IL-6水平均明显降低,差异有统计学意义(P<0.05);各用药组小鼠JAK2蛋白相对表达量均降低(P<0.05),XLT高剂量组和联合用药组p-JAK2降低差异有统计学意义(P<0.05);除PD-1抑制剂对p-STAT3蛋白的表达抑制作用不明显外,各用药组小鼠STAT3、p-STAT3蛋白相对表达量均较模型组显著降低(P<0.05)。结论升降理肺消瘤汤能够抑制Lewis肺癌�Objective To explore the regulatory effects of Shengjiang Lifei Xiaoliu Decoction(升降理肺消瘤汤,SJLFXLD)on immune and inflammatory responses in Lewis lung cancer mice,as well as its impact on the JAK2/STAT3 signaling pathway.Methods C57BL/6J male mice were fed adaptively for one week,with 8 in the blank group and 60 in the tumor bearing group.After successfully injecting Lewis lung cancer cells into the tumor bearing group mice for modeling,the mice whose tumor was visible to the naked eye were divided into a model group,a PD-1 inhibitor group,a low dose group of SJLFXLD,a medium dose group of SJLFXLD,a high dose group of SJLFXLD,and a combination of medium dose of SJLFXLD and PD-1 inhibitor group(combined medication group),with 8 mice in each group.The blank group and model group were given 0.4 mL/20 g normal saline by gavage every day.In the PD-1 inhibitor group,100μg of Sinilimab was injected intraperitoneally every 3 days.The low,medium and high dose groups of SJLFXLD were given 0.4 mL/20 g of corresponding concentration of Chinese herbal medicine by gavage every day.In the combined treatment group,0.4 mL/20 g of Chinese herbal medicine with medium concentration was given by gavage every day,and 100μg of Sinilimab was injected intraperitoneally every 3 days.Each group was continuously administered for 14 days.24 hours after the last administration,the weight of the mice was measured and recorded.Eyeballs were removed for blood collection,and the mice were euthanized using cervical dislocation method.The tumor inhibition rate,tumor removal body mass,thymus index,and spleen index of each group of mice were compared.ELISA method for detecting interleukin-2(IL-2)and interferon in serum-γ(IFN-γ),tumor necrosis factor-α(TNF-α),IL-6 concentration level.Western blot was used to detect the expression levels of Janus kinase 2(JAK2),p-JAK2,signal transducer and activator of transcription 3(STAT3),and p-STAT3 proteins.Results Compared with the model group,the average tumor weight of mice in each medication

关 键 词：升降理肺消瘤汤 LEWIS肺癌 免疫 炎症 细胞因子 JANUS激酶2 信号转导和转录激活因子3 

分 类 号：R285.5[医药卫生—中药学]