蛇黄凝胶与蛇黄散体外释放与透皮特性比较研究  

Comparative Study on Release and Transdermal Behavior in vitro between Shehuang Gels and Shehuang Powder

作　　者：汪亚飞 冯洁颖[1] 吴惠妃[1] 梅全喜 WANG Yafei;FENG Jieying;WU Huifei;MEI Quanxi(Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan,Guangdong 528401,China;Baoan Pure Traditional Chinese Medicine Hospital,Shenzhen,Guangdong 518101,China)

机构地区：[1]中山市中医院,广东中山528401 [2]宝安纯中医治疗医院,广东深圳518101

出　　处：《今日药学》2024年第2期106-111,共6页Pharmacy Today

基　　金：2022年度中山市医学科研项目(2022J006)。

摘　　要：目的比较蛇黄散与其改良剂型蛇黄凝胶的体外释放度与透皮特性。方法分别采用微孔滤膜与离体小鼠鼠皮及改进的Franz扩散池进行体外释放与透皮试验,以HPLC法定量测定蛇黄凝胶与蛇黄散中君药—蛇鳞草中triphyllin A与大黄中大黄酸的量为指标,测定2种制剂的体外释放量(Qs)、释放率(Q)、透皮量(Ls)与透皮率(L),并采用3种方程对蛇黄凝胶的体外释放与透皮结果进行模拟拟合。结果蛇黄凝胶中triphyllin A 24 h的Qs=72.1885μg·cm^(-2),Q=30.4583%,Ls=52.0867μg·cm^(-2),L=21.9768%,大黄酸24 h的Qs=15.2200μg·cm^(-2),Q=9.4290%,Ls=13.5456μg·cm^(-2),L=8.3917%,蛇黄散中triphyllin A 24 h的Qs=45.2395μg·cm^(-2),Q=17.1249%,Ls=27.0434μg·cm^(-2),L=10.2370%,大黄酸24 h的Qs=6.3763μg·cm^(-2),Q=1.9921%,Ls=5.8751μg·cm^(-2),L=1.8355%。结论改良后的蛇黄凝胶体外Qs、Q、Ls与L均优于蛇黄散,释放与透皮特征明显优于传统剂型蛇黄散,且蛇黄凝胶不易过敏,贴敷方便,便于临床使用;蛇黄凝胶释放与透皮模型均符合Higuchi方程,说明蛇黄凝胶体外释放和透皮均属于骨架型缓释制剂。OBJECTIVE To compare the release and transdermal behavior in vitro between Shehuang gels and Shehuang powder.METHODS Microporous fliter membrane and isolated rat skin and advanced Franz diffusion cell were chosen in the release and transdermal behavior in vitro between Shehuang gels and Shehuang powder.The content of triphyllin A in Pronephrium triphyllum and rhein in rheum of Shehuang gels and Shehuang powder were quantitatively determined by HPLC method to determine the penetration quantity and penetration rate of the two forms.The in vitro release and transdermal results of Shehuang gels were simulation fitting by 3equations.RESULTS The triphyllin A of Shehuang gels in 24 h were Qs=72.1885μg·cm^(-2),Q=30.4583%,Ls=52.0867μg·cm^(-2),L=21.9768%.The rhein of Shehuang gels in 24 h were Qs=15.2200μg·cm^(-2),Q=9.4290%,Ls=13.5456μg·cm^(-2),L=8.3917%.The triphyllin A of Shehuang powder in 24 h were Qs=45.2395μg·cm^(-2),Q=17.1249%,Ls=27.0434μg·cm^(-2),L=10.2370%.The rhein of Shehuang powder in 24 h were Qs=6.3763μg·cm^(-2),Q=1.9921%,Ls=5.8751μg·cm^(-2),L=1.8355%.CONCLUSION The Qs、Q、Ls and L of Shehuang gels are obviously better than the Shehuang powder.Meanwhile the Shehuang gels is less sensitized and more convenient to use in clinical.The release and transdermal model of Shehuang gels are coincides with Higuchi equation,the release and transdermal behavior of Shehuang gels are all belong to skeleton sustained-release preparations.

关 键 词：蛇黄凝胶 蛇黄散 triphyllin A 大黄酸 释放试验 透皮试验 

分 类 号：R28[医药卫生—中药学]