稻瘟病菌MoZds1的功能分析  

Functional study of MoZds1 in the rice blast fungus

作　　者：黄昌丽 朱学明 李琳[2] 鲍坚东[2] 俞晓平 林福呈 HUANG Changli;ZHU Xueming;LI Lin;BAO Jiandong;YU Xiaoping;LIN Fucheng(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310018 [2]农产品质量安全危害因子与风险防控国家重点实验室,浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2024年第3期559-568,共10页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金(LQ22C140005)。

摘　　要：由稻瘟病菌引起的水稻稻瘟病对中国水稻产量造成了严重威胁,开展稻瘟病菌功能基因的研究有利于更好地了解稻瘟病菌致病机制,为稻瘟病的防治奠定基础。研究前期通过遗传学手段在稻瘟病菌中筛选到一个酵母ZDS1同源基因MoZDS1。通过基因敲除方法成功获得基因缺失突变体ΔMozds1,发现缺失MoZDS1严重影响稻瘟病菌营养生长。为探究MoZds1在稻瘟病菌中的具体功能,通过生物信息学分析、生长测定、产孢量分析、细胞壁胁迫分析以及自噬流检测等方法,分析了MoZds1在维持营养生长、产孢、应对细胞壁胁迫以及细胞自噬方面的作用。结果表明,MoZds1正调控稻瘟病菌菌丝生长,负调控孢子的形成。此外,MoZds1参与细胞壁完整性途径并调控孢子内糖原的降解速率,然而,MoZds1的缺失未表现出对细胞自噬途径的显著影响。该研究对稻瘟病菌MoZds1的功能进行了系统地分析,或为后续更深入研究MoZds1的功能提供了理论基础。Rice blast disease caused by Magnaporthe oryzae has posed a serious threat to rice yield in China.Studying the gene functions of M.oryzae is beneficial for better understanding the pathogenic mechanism of M.oryzae and lays a foundation for the control of rice blast disease.In this study,a yeast ZDS1 homolog gene MoZDS1 was screened through genetic methods in M.oryzae.The gene deletion mutantΔMozds1 was successfully obtained by gene knockout method,and it was found that the deletion of MoZDS1 seriously affected the vegetative growth.In order to explore the functions of MoZds1,this study analyzed the roles of Mozds1 in maintaining vegetative growth,conidiation,response to cell wall stress and autophagy.The results showed that MoZds1 positively regulated the mycelium growth and negatively regulated the conidia production.In addition,MoZds1 was also involved in the cell wall integrity pathway and regulated the glycogen degradation in conidia,however,the deletion of MoZds1 did not show a significant effect on the autophagy pathway.Overall,this analysis of the function of MoZds1 systematically in M.oryzae,may provide a theoretical basis for further in-depth research on the function of MoZds1.

关 键 词：稻瘟病菌 气生菌丝 细胞壁完整性 细胞自噬 

分 类 号：Q786[生物学—分子生物学]