国槐枝枯病病原菌鉴定及其生物学特性  

Identification and biological characteristics of pathogen causing branch dieback on Styphnolobium japonicum(L.)Schott

作　　者：罗芷涵 刘朋飞 于军[2] 齐鹤 陈小光 楼兵干[1] LUO Zhihan;LIU Pengfei;YU Jun;QI He;CHEN Xiaoguang;LOU Binggan(Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insects,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China;College of Plant Science,Tarim University,Alar 843399,Xinjiang,China;Forestry and Grassland Administration of Bayingol Mongolian Autonomous Prefecture,Korla 841000,Xinjiang,China;Landscaping Affair Center of Korla,Korla 841000,Xinjiang,China)

机构地区：[1]浙江大学生物技术研究所,农业农村部作物病虫分子生物学重点实验室,浙江杭州310058 [2]塔里木大学植物科学学院,新疆阿拉尔843399 [3]巴音郭楞蒙古自治州林业和草原局,新疆库尔勒841000 [4]库尔勒市园林局,新疆库尔勒841000

出　　处：《浙江农业学报》2024年第3期579-588,共10页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划(2021YFD1400200)。

摘　　要：近年来,枝枯病在我国南疆的发病率高达50%~70%。为明确国槐枝枯病的致病菌及其生物学特性,采用组织分离法对南疆多地的发病国槐枝条进行病原菌分离,并进行致病性测定、形态学和分子生物学鉴定。结果表明,引起国槐枝枯病的病原菌为新暗色柱节孢(Neoscytalidium dimidiatum)。PDA培养基(pH值5.0)、34℃条件最适宜该菌的菌丝生长;PDA培养基(pH值7.0~9.0)、34℃条件最适宜该菌的分生孢子的形成。较低的相对湿度更有利于该菌的分生孢子萌发和生长。以牛肉膏为氮源、甘露糖为碳源最有利于该菌的菌丝生长,以蛋白胨为氮源最适宜该菌分生孢子的产生;而以木糖、山梨糖为碳源,硝态氮、尿素为氮源时,菌丝生长及产孢速率均有所下降。Branch dieback of Styphnolobium japonicum(L.)Schott is a serious disease that has occurred in the southern Xinjiang in recent years.To identify the pathogen of branch dieback in S.japonicum and its biological characteristics,we collected the samples with typical symptoms and the pathogen were isolated and purified.Based on the pathogenicity tests,morphological and molecular identification,we confirmed that the pathogen is Neoscytalidium dimidiatum.The results of biological tests showed that the optimal conditions for mycelial growth of N.dimidiatum were PDA medium(pH 5.0)at 34℃.The spore production was optimal on PDA medium(pH 7.0-9.0)at 34℃.The best C&N sources for mycelial growth were mannose and beef extract,respectively,while peptone was best for sporulation.In contrast,mycelial growth and spore production rate decreased when xylose and sorbose were used as carbon sources and nitrate nitrogen and urea as nitrogen sources.

关 键 词：国槐枝枯病 病原鉴定 新暗色柱节孢(Neoscytalidium dimidiatum) 生物学特性 

分 类 号：S763.7[农业科学—森林保护学]