基于网络药理学和动物实验探究利咽方治疗慢性咽炎的机制  

Exploring the Mechanism of Liyan Formula in the Treatment of Chronic Pharyngitis Based on Network Pharmacology and Animal Experiments

作　　者：曹雅岚 李淑坤 黄一平[1,2] 顾晓娜[1,2] 张鹏[1,2] CAO Yalan;LI Shukun;HUANG Yiping;GU Xiaona;ZHANG Peng(Hospital of Integrated Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Province Hospital on Integration of Chinese and Western Medicine,Nanjing 210028,China)

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210023 [2]江苏省中西医结合医院,江苏南京210028

出　　处：《中医药信息》2024年第3期25-33,共9页Information on Traditional Chinese Medicine

基　　金：2023年中央财政转移支付项目(2023ZYCZ-002)。

摘　　要：目的:基于网络药理学和动物实验,探讨利咽方治疗慢性咽炎的作用机制。方法:从TCMSP数据库中收集利咽方中的有效成分及作用靶点,从GeneCards数据库中收集慢性咽炎的相关靶点,然后通过Venny和STRING网站得到交集靶点,对交集靶点进行KEGG通路分析和Gene Ontology富集分析。并采用Tongg氨水直接喷雾法建立大鼠慢性咽炎模型对上述网络药理学的结果进行验证。结果:共收集利咽方中141个活性化合物和205个作用靶点以及1950个疾病靶点,取交集得到112个靶点,交集靶点的PPI网络分析得到IL-6、TNF和IL-β等重要靶点。KEGG通路显示,与利咽方治疗慢性咽炎关联度较高的通路为癌症相关通路。GO富集分析结果表明,利咽方的治疗作用生物过程主要涉及无机物反应;细胞组分主要涉及膜筏、细胞器外膜、囊腔等位置;分子功能主要涉及细胞因子受体结合、DNA转录因子结合等功能。动物实验结果显示,利咽方能有效降低血清中TNF-α、IL-1β、IL-6的水平,降低大鼠咽喉黏膜组织中TLR-4、NF-κBp65及磷酸化I-κBα蛋白水平。结论:通过网络药理学预测了利咽方的有效成分及其治疗慢性咽炎的潜在靶点,同时进行的动物实验表明利咽方可通过调节TLR-4/NF-κB信号通路,治疗氨水诱导的大鼠慢性咽炎。Objective:To explore the mechanism of Liyan Formula in the treatment of chronic pharyngitis based on network pharmacology and animal experiments.Methods:The active components and targets of Liyan Formula were collected from TCMSP database and related targets of chronic pharyngitis were collected from the GeneCards database.Intersection targets were obtained through Venny and String websites,and KEGG pathway analysis and Gene Ontology enrichment analysis were performed on the intersection targets.Tongg ammonia spray was used to establish a rat model of chronic pharyngitis,and the results of network pharmacology were verified.Results:141 active compounds,205 action targets and 1950 disease targets were collected on Liyan Formula,and 112 targets were obtained by intersection analysis.Important targets such as IL-6,TNF and IL-βwere obtained by PPI network analysis of intersection targets.KEGG pathway showed that the pathway with high correlation with Liyan Formula in treating chronic pharyngitis was cancer-related pathway.The results of GO enrichment analysis showed that the biological process of the therapeutic effect of Liyan Formula mainly involved the reaction of inorganic substances.The cell components mainly involved positions such as the membrane raft,the outer membrane of the organelle,and the cyst cavity.The molecular functions were mainly related to cytokine receptor binding and DNA transcription factor binding.The results of animal experiments showed that Liyan Formula could effectively reduce the levels of TNF-α,IL-1βand IL-6 in serum,and reduce the levels of TLR-4,NF-κB P65 and phosphorylated I-κBαprotein in rat throat mucosa.Conclusion:The active components of Liyan Formula and its potential targets for the treatment of chronic pharyngitis are predicted through network pharmacology.Meanwhile,the animal experiments showed that Liyan Formula can treat ammonia-induced chronic pharyngitis in rats by regulating TLR-4/NF-κB signaling pathway.

关 键 词：慢性咽炎 利咽方 网络药理学 TLR-4/NF-κB信号通路 

分 类 号：R285.5[医药卫生—中药学]