高效液相色谱-电喷雾检测器法同时测定心荣颗粒的多种成分  

Simultaneous Determination of Several Constituents in XinRong Granules by HPLC-CAD

作　　者：李玥 傅琳[2] LI Yue;FU Lin(Dagang Hospital of Traditional Chinese Medicine of Tianjin Binhai New Area,Tianjin 300000,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300301,China)

机构地区：[1]天津市滨海新区大港中医医院,天津300000 [2]天津药物研究院,天津300301

出　　处：《海峡药学》2024年第3期41-44,共4页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱-电雾式检测器法同时测定心荣颗粒中黄芪甲苷、麦冬皂苷D、地黄苷D、五味子醇甲、芍药苷、桂皮醛的含量。方法色谱柱为Atlantis PREMIER BEH C 18色谱柱(250 mm×4.6 mm,5μm);流动相以甲醇(A)-0.1%乙酸水溶液(B)梯度洗脱,柱温35℃,流速1 mL·min^(-1),进样量10μL。电喷雾检测器的雾化器温度为35℃,检测频率为10 Hz。结果黄芪甲苷、麦冬皂苷D、地黄苷D、五味子醇甲、芍药苷、桂皮醛在本文浓度范围内均呈良好线性关系,且平均加样回收率分别为黄芪甲苷98.9%(RSD为0.8%,n=9)、麦冬皂苷D 98.4%(RSD为1.4%,n=9)、地黄苷D 98.7%(RSD为1.4%,n=9)、五味子醇甲99.4%(RSD为0.7%,n=9)、芍药苷100.1%(RSD为0.9%,n=9)、桂皮醛101.3%(RSD为0.6%,n=9)。结论该方法可用于心荣颗粒中黄芪甲苷、麦冬皂苷D、地黄苷D、五味子醇甲、芍药苷、桂皮醛的含量测定。OBJECTIVE To establish a HPLC-CAD method for simultaneously determination the contents of Astragaloside IV,Ophiopogonin D,Rehmannioside D,Schizandrin,Paeoniflorin,Cinnamaldehyde in XinRong granules.METHODS The analysis was conducted on the Atlantis PREMIER BEH C_(18)(4.6×250 mm,5μm),which maintained at 35℃column temperature,methanol(a)-0.1%acetic acid aqueous solution(b)was the mobile phase in a gradient elution mode while the flow rate was 1.0 mL·min^(-1),the nebulization temperature of the detector was 35℃,and the injection volume was 10μL.RESULTS The linear ranges of Astragaloside IV,Ophiopogonin D,Rehmannioside D,Schizandrin,Paeoniflorin,Cinnamaldehyde were good.The average recoveries of the six components were 98.9%,98.4%,98.7%,99.4%,100.1%,101.3%;and the RSD were 0.8%,1.4%,1.4%,0.7%,0.9%,0.6%respectively.CONCLUSION The method can be used for the determination of XinRong granules.

关 键 词：心荣颗粒 电雾式检测器 黄芪甲苷 麦冬皂苷D 地黄苷D 五味子醇甲 芍药苷 桂皮醛 

分 类 号：R927[医药卫生—药学]