紫外-可见分光光度法测定舒筋消肿散中双酯型生物碱限量  

Determination of Diester Alkaloids in ShuJin XiaoZhong Powder by Uleraviolet Visible Spectrophotometry

作　　者：叶希奇 吴军军 潘丕阑 YE Xi-qi;WU Jun-jun;PAN Pi-lang(Hospital of Traditional Chinese in Ningde affiliated to Fujian University of Tranditonal Chinese Medicine,Ningde 352000,China)

机构地区：[1]福建中医药大学附属宁德中医院,福建宁德352100

出　　处：《海峡药学》2024年第3期48-51,共4页Strait Pharmaceutical Journal

摘　　要：目的建立舒筋消肿散中双酯型生物碱的限量测定方法。方法应用紫外-可见分光光度法,采用改良盐酸羟胺-高氯酸铁法显色,在520 nm波长处测定乌头碱对照液和供试品溶液的吸光度,计算供试品溶液中双酯型生物碱的含量。结果乌头碱对照液浓度在23.1~231.0μg·mL^(-1)范围内线性关系良好,回归方程A=0.00705C+0.000134,r=0.999891。乌头碱平均回收率92.42%,RSD=1.20%(n=9)。测得10批舒筋消肿散中双酯型生物碱限量,以乌头碱计分别为:0.131%、0.065%、0.077%、0.117%、0.108%、0.081%、0.097%、0.122%、0.103%、0.092%。结论建立的测定方法简便、准确、灵敏、重现性良好,适用于舒筋消肿散中双酯型生物碱限量的测定。OBJECTIVE Establish the limit method of diester alkaloids in ShuJin XiaoZhong powder.METHODS Determination of absorbance of the aconitine solution and the test solution at 520 nm and calculate the content of diester alkaloids in the test solution adoption of ulteraviolet visible spectrophotometry and modified hydroxylamine hydrochloride-iron perchlorate color process.RESULTS There was a good linearity within the limit of aconitine during 23.1-231.0μg·mL^(-1).The equation of regression was A=0.00705C+0.000134,r=0.999891.The average recovery of aconitine was 92.42%,RSD=1.20%(n=9).The limits of diester alkaloids in 10 batches of ShuJin XiaoZhong powder were 0.131%,0.065%,0.077%,0.117%,0.108%,0.081%,0.097%,0.122%,0.103%,0.092%according to aconitine respectively.CONCLUSION The established method is simple,accurate,sensitive and reproducible.It is suitable for the determination of diester alkaloids in ShuJin XiaoZhong powder.

关 键 词：舒筋消肿散 紫外-可见分光光度法 双酯型生物碱限量 

分 类 号：R927[医药卫生—药学]