苦参碱对骨肉瘤细胞凋亡的影响  

Effect of Matrine on Apoptosis of Osteosarcoma Cells

作　　者：王利宁 赵新芝[2] 宋军帅 王淑燕[3] 孙广玉[3] 李克明[2] 杨真真 WANG Lining;ZHAO Xinzhi;SONG Junshuai;WANG Shuyan;SUN Guangyu;LI Keming;YANG Zhenzhen(Rushan People's Hospital,Weihai,Shandong,China 264599;Shandong Academy of Traditional Chinese Medicine,Jinan,Shandong,China 250014;Binzhou People's Hospital,Binzhou,Shandong,China 256600;The Fourth People's Hospital of Jinan City,Jinan,Shandong,China 250031)

机构地区：[1]山东省乳山市人民医院,山东威海264599 [2]山东省中医药研究院,山东济南250014 [3]山东省滨州市人民医院,山东滨州256600 [4]山东省济南市第四人民医院,山东济南250031

出　　处：《中国药业》2024年第7期42-47,共6页China Pharmaceuticals

基　　金：山东省中医药科技项目[2021M191];青岛大学医疗集团科研专项[YLJT20212020]。

摘　　要：目的探讨苦参碱在MG-63细胞中促凋亡的作用及机制。方法实验分为0浓度组和10%苦参碱组,分别灌胃生理盐水和10%苦参碱制备大鼠含药血清,干预人成骨肉瘤细胞株MG-63。采用CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,荧光定量聚合酶链式反应(PCR)检测c-myc、胱天蛋白酶9(caspase-9)基因的表达,采用免疫印迹(Western blot)法检测细胞外信号调节激酶5(ERK5)信号通路中相关蛋白Nur77、丝裂原激活蛋白激酶5(MEK5)及凋亡相关蛋白c-myc,caspase-9的表达水平。结果与0浓度组相比,10%苦参碱组能显著抑制MG-63细胞增殖,并促进其凋亡;荧光定量PCR实验结果表明,10%苦参碱组能抑制c-myc转录,并促进caspase-9转录;Western blot实验结果表明,在ERK5信号通路中,10%苦参碱组Nur77、MEK5及c-myc蛋白表达下调,而caspase-9蛋白表达上调。结论苦参碱可能通过干扰ERK5信号通路,调控c-myc,caspase-9等蛋白的表达,从而抑制MG-63细胞增殖,并促进其凋亡。Objective To investigate the effect and mechanism of matrine in promoting apoptosis in MG-63 cells.Methods The experiment was divided into the non-matrine group and the 10% matrine group,and the rats in the two groups were orally administered normal saline and 10% matrine respectively to prepare drug-contained serum,and human osteosarcoma cell line MG-63 was intervened.CCK-8 method was used to detect cell proliferation.Flow cytometry was used to detect apoptosis.Fluorescence quantitative PCR was used to detect the expression of c-myc and caspase-9 genes.The expressions of related proteins Nur77,mitogen-activated protein kinase 5(MEK5),and apoptosis-related proteins c-myc and caspase-9 in extracellular signal-regulated kinase 5(ERK5)signaling pathway were detected by Western blot.Results Compared with the non-matrine group,the proliferation of MG-63 cells were significantly inhibited and their apoptosis were promoted in the 10% matrine group.The results of fluorescence quantitative PCR experiments showed that 10% matrine could inhibit c-myc transcription and promote caspase-9 transcription.Western blot experiments showed that in the ERK5 signaling pathway,10% matrine could downregulate the expression of Nur77,mitogen-activated protein kinase 5(MEK5),and c-myc protein,and upregulate the expression of caspase-9 protein.Conclusion Matrine may inhibit the proliferation of MG-63 cells and promote their apoptosis through interfering with the ERK5 signaling pathway and regulating the expression of proteins such as c-myc and caspase-9.

关 键 词：苦参碱 骨肉瘤 MG-63细胞 细胞外信号调节激酶5信号通路 凋亡 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]