2012年-2022年TBTV、TVDV、TBTVsatRNA和TVDVaRNA在云南主要烟区的发生与分布  

Occurrence and distribution of TBTV,TVDV,TBTVsatRNA and TVDVaRNA in main tobacco planting areas of Yunnan province from 2012 to 2022

作　　者：杨亚辉 杨洁[1] 罗恒明 刘芳[1] 吕婧 陈平 陈海如[1] 陈小姣 李凡[1] YANG Yahui;YANG Jie;LUO Hengming;LIU Fang;L Jing;CHEN Ping;CHEN Hairu;CHEN Xiaojiao;LI Fan(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201

出　　处：《植物保护》2024年第2期296-302,331,共8页Plant Protection

基　　金：国家自然科学基金(31960532,31701767,31571983);云南省院士专家工作站(202005AF150040)。

摘　　要：烟草丛顶病(tobacco bushy top disease,TBTD)是一种由烟草丛顶病毒(tobacco bushy top virus,TBTV)及其卫星RNA(tobacco bushy top virus satellite RNA,TBTVsatRNA)和烟草扭脉病毒(tobacco vein distorting virus,TVDV)及其伴随RNA(tobacco vein distorting virus associated RNA,TVDVaRNA)共同侵染引起的危险性病害,曾在云南很多烟区造成严重损失。本研究对烟草丛顶病及其相关病原物在云南烟草上的发生情况进行了多年的调查研究,力图对烟草丛顶病及其相关病原物的发生与分布进行追踪和分析。结果表明,2012年-2022年,烟草丛顶病在云南各大烟区仅零星发生,2019年以来已很难发现。烟草丛顶病的相关病原物主要在曾经暴发流行过病害的楚雄、保山和大理发现,且TBTV、TVDV、TBTVsatRNA和TVDVaRNA在烟草上并不总是同时被检出,其中引起烟草丛顶病典型症状的TBTV+TVDV+TBTVsatRNA+TVDVaRNA检出率在5%以下,TVDV的检出率最高,达30.44%,TBTVsatRNA的检出率最低,为7.68%。TBTV、TVDV、TBTVsatRNA和TVDVaRNA不能同时感染同一烟株可能是当前烟草丛顶病很少发生的主要原因。Tobacco bush top disease(TBTD)is a dangerous disease caused by a combination of tobacco bushy top virus(TBTV)and its satellite RNA(TBTVsatRNA),tobacco vein distorting virus(TVDV)and its associated RNA(TVDVaRNA).TBTD has caused serious losses in the main tobacco-planting areas in Yunnan province during the past decades.In order to track the occurrence and distribution of TBTD and its causal agents,disease survey and virus detection were conducted in this study.The results showed that TBTD occurred sporadically during 2012 to 2022,and no TBTD was found since 2019.TBTD causal agents mainly occurred in Chuxiong,Baoshan and Dali,where the disease recurred several times in 1990s.TBTV,TVDV,TBTVsatRNA and TVDVaRNA were not always detected on the same tobacco plant,and the detection rate of TBTV+TVDV+TBTVsatRNA+TVDVaRNA,which caused typical symptoms of TBTD,was less than 5%,TVDV showed the highest detection rate of 30.44%,while TBTVsatRNA had the lowest rate of 7.68%.The fact that TBTV,TVDV,TBTVsatRNA and TVDVaRNA failed to simultaneously infect the same tobacco plant may be the main reason why tobacco bushy top disease rarely occurs at present.

关 键 词：烟草丛顶病毒 烟草扭脉病毒 烟草丛顶病毒卫星RNA 烟草扭脉病毒伴随RNA 发生与分布 

分 类 号：S432.41[农业科学—植物病理学]