改良HPLC法测定利可君含量  

Determination of Leucongen by Improved HPLC

作　　者：董飞 欧阳臻[1] 张小平 陈进 曹旭 Dong Fei;Ouyang Zhen;Zhang Xiaoping;Chen Jin;Cao Xu(School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;Jiangsu Jibeier Pharmaceutical Co.,Ltd.,Zhenjiang 212009,China)

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏吉贝尔药业股份有限公司,江苏镇江212009

出　　处：《山东化工》2024年第4期156-158,162,共4页Shandong Chemical Industry

摘　　要：目的:建立一种改良后的HPLC法用于利可君含量测定。方法:采用高效液相色谱法,色谱柱:赛默飞C 18(4.6 mm×100 mm,3μm),流动相:水-乙腈-冰醋酸(体积比75∶25∶0.3),等度洗脱,柱温:30℃,检测波长:210 nm,进样量:20μL,流速:1.0 mL/min。结果:利可君的四个非对映异构体在HPLC中能全部检出且分离彻底,利可君在11.78~294.50μg/mL范围内线性良好,精密度、重现性优,平均回收率为98.48%。结论:本方法专属性好、灵敏度高、分离度高、理论塔板数高,适用于利可君的含量测定。Objective:To establish an improved HPLC method to determine the content of Leucongen.Methods:The isocratic elution HPLC method was used.Thermo Scientific TM C 18(4.6 mm×100 mm,3μm)was adopted with a mobile phase consisting of water-acetonitrile-acetic acid,glacial(75∶25∶0.3).The column temperature was 30℃ and wavelength was 210 nm.The injection volume was 20μL and flow rate was 1.0 mL/min.Results:The four diastereomers of leucogen can be detected and separated completely by HPLC.There was a good linear relationship in the range of 11.78~294.50μg/mL.It had good precision and reproducibility.The average recoveries was 98.48%.Conclusion:The method had good specificity and higher sensitivity,resolution,number of theoretical plates,it was suited to determine the content of Leucongen.

关 键 词：改良 利可君 含量测定 高效液相色谱法 

分 类 号：TQ460[化学工程—制药化工]