腹部推拿对溃疡性结肠炎大鼠脊髓背角PI3K、NR1表达及神经元形态的影响  

Effects of Abdominal Tuina on PI3K, NR1 Expression and Neuronal Morphology of Spinal Dorsal Horn in Rats with Ulcerative Colitis

作　　者：方佳钰 江煜 林志刚 陈水金 陈乐春 张幻真 蒋晶晶 陈进城 FANG Jiayu;JIANG Yu;LIN Zhigang;CHEN Shuijin;CHEN Lechun;ZHANG Huanzhen;JIANG Jingjing;CHEN Jincheng(Fujian University of Traditional Chinese Medicine,Fuzhou 350100,China;Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;Fujian Provincial Key Laboratory of Rehabilitation Technology,Fuzhou 350003,China)

机构地区：[1]福建中医药大学,福建福州350100 [2]福建中医药大学附属康复医院,福建福州350003 [3]福建省康复技术重点实验室,福建福州350003

出　　处：《中国中医药信息杂志》2024年第4期118-123,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(82004494、82174523、82105039);福建省自然科学基金(2022J01882、2020J01757、2020J01758);福建省卫生健康科技项目(2020GGA070、2020CXA052);福建省康复技术重点实验室课题(KF2019021)。

摘　　要：目的观察腹部推拿对溃疡性结肠炎(UC)大鼠脊髓背角磷脂酰肌醇-3-激酶(PI3K)、N-甲基-D-天冬氨酸受体(NMDAR)亚基NR1表达及神经元形态的影响,探讨其治疗UC的作用机制。方法36只SD大鼠随机分为正常组、模型组、腹部推拿组、美沙拉嗪组、PI3K激动组和PI3K激动+腹部推拿组,每组6只。采用5%葡聚糖硫酸钠溶液自由饮用建立UC大鼠模型,腹部推拿组和PI3K激动+腹部推拿组予腹部推拿干预,美沙拉嗪组予美沙拉嗪溶液灌胃,PI3K激动组和PI3K激动+腹部推拿组鞘内注射PI3K激动剂,均1次/d,连续15 d。腹壁撤退反射(AWR)评分及乙酸扭体反应观察大鼠腹痛症状,免疫荧光染色和Western blot检测大鼠脊髓背角PI3K、NR1表达,尼氏染色观察脊髓背角神经元形态。结果与正常组比较,模型组大鼠AWR评分及扭体次数显著增加(P<0.01),脊髓背角PI3K、NR1蛋白表达显著升高(P<0.05,P<0.01),脊髓背角神经元形态紊乱、形成大量空泡样结构,尼氏体结构模糊、不完整。与模型组比较,腹部推拿组和美沙拉嗪组大鼠AWR评分及扭体次数显著减少(P<0.05,P<0.01),脊髓背角PI3K、NR1蛋白表达显著降低(P<0.05,P<0.01),脊髓背角神经元水肿较轻、空泡样改变较少,尼氏体数量增加;PI3K激动组和PI3K激动+腹部推拿组AWR评分及扭体次数显著增加(P<0.05,P<0.01),脊髓背角PI3K、NR1蛋白表达显著升高(P<0.05,P<0.01),大量神经元固缩坏死,尼氏体数量减少,甚至溶解消失。结论腹部推拿能有效改善UC模型大鼠腹痛症状,其作用机制可能与抑制脊髓背角PI3K、NR1表达,改善脊髓背角神经元形态有关。Objective To investigate the effects of abdominal tuina on the expression of PI3K and N-methyl-D-aspartate receptor(NMDAR)subunit NR1 in spinal dorsal horn and the morphology of spinal dorsal horn neurons in ulcerative colitis(UC)rats;To explore its mechanism of action in treating UC.Methods Totally 36 SD rats were randomly divided into normal group,model group,abdominal tuina group,mesalazine group,PI3K stimulation group and PI3K stimulation+abdominal tuina group,with 6 rats in each group.The UC model in rats was simulated by drinking dextran sulfate solution freely.The abdominal tuina group and the PI3K stimulation+abdominal tuina group were given abdominal tuina intervention,the mesalazine group was given mesalazine solution for gavage,and the PI3K stimulation group and PI3K stimulation+abdominal tuina group were given intrathecal injection of PI3K agonist,once a day,for consecutive 15 days.Abdominal withdrawal reflex(AWR)score and acetic acid twist were used to observe the abdominal pain symptoms in rats.The expression of PI3K and NR1 in spinal dorsal horn were detected by immunofluorescence staining and Western blot,and the morphological changes of spinal dorsal horn neurons were observed by Nissl staining.Results Compared with the normal group,AWR score and twisting times of rats in model group significantly increased(P<0.01),the expression of PI3K and NR1 protein in spinal dorsal horn significantly increased(P<0.05,P<0.01),the morphology of spinal dorsal horn neurons was disordered,forming a large number of vacuolar like structures,and the Nissl body structure was fuzzy and incomplete.Compared with the model group,AWR scores and twisting times of abdominal tuina group and mesalazine group significantly decreased(P<0.05,P<0.01),and the expression of PI3K and NR1 protein significantly decreased(P<0.05,P<0.01),the edema of spinal dorsal horn neurons was milder,with fewer vacuolar changes and an increase in the number of Nissl bodies;AWR scores and twisting times of PI3K stimulation group and PI3K stimulation+

关 键 词：腹部推拿 溃疡性结肠炎 脊髓背角 磷脂酰肌醇-3-激酶 N-甲基-D-天冬氨酸受体 

分 类 号：R244.1[医药卫生—针灸推拿学]