Composition,function,and timing:exploring the early‑life gut microbiota in piglets for probiotic interventions  

作　　者：Jianping Quan Cineng Xu Donglin Ruan Yong Ye Yibin Qiu Jie Wu Shenping Zhou Menghao Luan Xiang Zhao Yue Chen Danyang Lin Ying Sun Jifei Yang Enqin Zheng Gengyuan Cai Zhenfang Wu Jie Yang 

机构地区：[1]College of Animal Science and National Engineering Research Center for Breeding Swine Industry,South China Agricultural University,Guangzhou,Guangdong,People’s Republic of China [2]Guangdong Provincial Key Laboratory of Agro‑Animal Genomics and Molecular Breeding,South China Agricultural University,Guangzhou,Guangdong,China [3]Yunfu Subcenter of Guangdong Laboratory for Lingnan Modern Agriculture,Yunfu,Guangdong,China [4]National Engineering Research Center for Breeding Swine Industry,Wens Foodstuff Group Co.,Ltd.,Yunfu,Guangdong,People’s Republic of China

出　　处：《Journal of Animal Science and Biotechnology》2024年第2期659-678,共20页畜牧与生物技术杂志（英文版）

基　　金：supported by a Key Technologies R&D Program of Guangdong Province project(2022B0202090002);a China Postdoctoral Science Foundation(Grant No.2021M701263);a Local Innovative and Research Teams Project of Guangdong Province(2019BT02N630);a Project of Swine Innovation Team in the Guangdong Modern Agricultural Research System(2023KJ126).

摘　　要：Background The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous effects.However,the optimal timeframe for introducing probiotics to achieve this outcome remains uncertain.Results In the context of this investigation,we conducted a longitudinal assessment of the fecal microbiota of 63 piglets at three distinct pre-weaning time points.Simultaneously,we gathered vaginal and fecal samples from 23 sows.Employing 16S rRNA gene and metagenomic sequencing methodologies,we conducted a comprehensive analysis of the fluctuation patterns in microbial composition,functional capacity,interaction networks,and colonization resistance within the gut microbiota of piglets.As the piglets progressed in age,discernible modifications in intestinal microbial diversity,composition,and function were observed.A source-tracking analysis unveiled the pivotal role of fecal and vaginal microbiota derived from sows in populating the gut microbiota of neonatal piglets.By D21,the microbial interaction network displayed a more concise and efficient configuration,accompanied by enhanced colonization resistance relative to the other two time points.Moreover,we identified three strains of Ruminococcus sp.at D10 as potential candidates for improving piglets’weight gain during the weaning phase.Conclusions The findings of this study propose that D10 represents the most opportune juncture for the introduction of external probiotic interventions during the early stages of piglet development.This investigation augments our comprehension of the microbiota dynamics in early-life of piglets and offers valuable insights for guiding forthcoming probiotic interventions.

关 键 词：Colonization resistance Intestinal microbiota PIGLET WEANING Weight gain 

分 类 号：S828[农业科学—畜牧学]