高效液相色谱-蒸发光散射检测法测定戊二胺的成分含量  

High Performance Liquid Chromatography-evaporative Light Scattering Detection Method to Determine the Component Content of Pentanediamine

作　　者：杨梦茜 穆玉敏 张浩月 刘丽荣 翟宇杰 任丽梅 Yang Mengxi;Mu Yumin;Zhang Haoyue;Liu Lirong;Zhai Yujie;Ren Limei(Department of Chemical Engineering,Shijiazhuang University,Shijiazhuang 050035,China;Hebei Provincial University Microbiology Pharmaceutical Application Technology Research and Development Center,Shijiazhuang University,Shijiazhuang 050035,China;Hebei International Joint Research Center for Biopharmaceutical,Shijiazhuang University,Shijiazhuang 050035,China;Mei bang mei he Biological Technology Corporation Ltd.,Shijiazhuang 050035,China)

机构地区：[1]石家庄学院化工学院,河北石家庄050035 [2]石家庄学院河北省高校微生物制药应用技术研发中心,河北石家庄050035 [3]石家庄学院河北省生物制药国际联合研究中心,河北石家庄050035 [4]美邦美和生物科技有限公司,河北石家庄050035

出　　处：《广东化工》2024年第3期143-145,153,共4页Guangdong Chemical Industry

摘　　要：利用反向液相色谱串联蒸发光检测器开发建立了一种新的检测戊二胺分析方法。蒸发光检测器通用性好,克服了戊二胺无紫外及可见光吸收特性所带来的的缺点。采用依利特C18色谱柱分离,甲醇-水进行洗脱,蒸发光检测器进行检测,以外标法定量。通过此方法,在10 min内可以实现戊二胺及其原料L-赖氨酸的分离与含量测定。此两种物质的分离度可达到2以上,戊二胺的线性范围(94.5~1890)μg/mL,L-赖氨酸的线性范围(95~1890)μg/mL,相关系数(r)为0.9992~0.9999,线性关系良好,3个不同浓度水平的加标实验表明,戊二胺的回收率为97.05%~100.16%,RSD值为0.42%~1.46%。此方法操作简单,灵敏度高准确性好,测试效率高,适用于生物合成戊二胺及尼龙56等过程中的分析检测。A new analytical method for the detection of pentanediamine was developed by reverse liquid chromatography tandem evaporative light detector.The evaporative light detector has good versatility and overcomes the shortcomings caused by the absence of ultraviolet and visible light absorption characteristics of pentanediamine.C18 column separation,methanol-water for elution and evaporative light detector for detection,external standard quantification.By this method,the separation and content determination of pentanediamine and its raw material L-lysine can be realized within 10 minutes.The resolution of these two substances can reach more than 2,the linear range of pentanediamine is(94.5~1890)μg/mL,the linear range of L-lysine is(95~1890)μg/mL,the correlation coefficient(r)is 0.9992~0.9999,the linear relationship is good,and the spike experiments of three different concentration levels show that the recovery rate of pentanediamine is 97.05%~100.16%,and the RSD value is 0.42%~1.46%.This method is simple to operate,has high sensitivity,good accuracy and high test efficiency,and is suitable for analytical detection in the process of biosynthesis of pentanediamine and nylon 56.

关 键 词：戊二胺 生物合成 蒸发光散射 外标法 尼龙56 

分 类 号：O657[理学—分析化学]